Custom Adeno-Associated Virus (AAV) Service

Recombinant Adeno-Associated Virus (AAV) is the most promising candidate for gene therapy among all viral vectors owing to its non-integrating nature and lack of pathogenicity in animal models. It is also a powerful tool in regenerative medicine due to its broad tropism and ability to transduce both proliferating and quiescent cells.

Tissue specificity of AAV can be achieved through the use of different AAV serotype capsids. abm offers packaged AAV virus as purified stock, ideal for in vivo experiments, or a crude virus preparation for in vitro cell culture experiments. Whether you are looking for over-expression or knockdown, we have it all!

Advantages of AAV:

  • Does not elicit significant immune responses in vivo
  • Broad tropism - tissue specificity with different AAV serotypes
  • No integration into the host genome
  • Ability to transduce both proliferating and quiescent cells
  • Long-term expression in non-dividing cells



Our AAV infectivity Data Speaks for Itself

Workflow
Left: EGFP expression (Green) in lumbar neuronal cells 4 weeks after intrathecal injection of AAV-EGFP Serotype 9 (Cat. # iAAV01509) into mice.
Right: Overlay with β-tubulin (red) and DAPI (blue).
Image courtesy of Dr. Douglas Lopes, King’s College London.
Testimonial:

"If I have any special needs or concerns, they are always dealt with by the Technical Support Team. I have always been pleased with the product and will continue to use this service for my research."

John Teem, Florida Department of Agriculture & Consumer Services

Additional Resources:

Service Details


Service Selection Assistant & Services List
How are you going to provide your insert?

What titer would you like to produce?


What serotype would you like to use?


Gene Synthesis
Service NameSizeCAT. NO.PRICE
Gene Synthesis Per bp C098 USD$0.18
*minimum charge $99; maximum size 3kb for AAV.
Custom AAV Plasmid Cloning
Service NameSizeCAT. NO.PRICE
Custom AAV plasmid cloning with compatible insert 1 Service C234 $350.00
Custom AAV plasmid cloning without compatible insert 1 Service C235 $450.00

*For these services, the customer must provide a gene insert under 3kb. Please contact [email protected] for more information on available cloning vectors and restriction enzyme sites to qualify for the compatible insert service.

Custom AAV Production without DNA Amplification
Service NameSizeCAT. NO.PRICE
Custom AAV without DNA amplification (10^9 GC/ml) (AAV1) 5 x 200 μl C239 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV2) 5 x 200 μl C240 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV3) 5 x 200 μl C290 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV4) 5 x 200 μl C291 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV5) 5 x 200 μl C241 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV6) 5 x 200 μl C242 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV7) 5 x 200 μl C243 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV8) 5 x 200 μl C244 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV9) 5 x 200 μl C245 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV10) 5 x 200 μl C246 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAV11) 5 x 200 μl C344 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAVDJ) 5 x 200 μl C345 $400.00
Custom AAV without DNA amplification (10^9 GC/ml) (AAVDJ-8) 5 x 200 μl C346 $400.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV1) 2 x 100 μl C247 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV2) 2 x 100 μl C248 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV3) 2 x 100 μl C292 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV4) 2 x 100 μl C293 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV5) 2 x 100 μl C249 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV6) 2 x 100 μl C250 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV7) 2 x 100 μl C251 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV8) 2 x 100 μl C252 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV9) 2 x 100 μl C253 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV10) 2 x 100 μl C254 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV11) 2 x 100 μl C488 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAVDJ) 2 x 100 μl C489 $950.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAVDJ-8) 2 x 100 μl C490 $950.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV1) 2 x 100 μl C255 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV2) 2 x 100 μl C256 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV3) 2 x 100 μl C294 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV4) 2 x 100 μl C295 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV5) 2 x 100 μl C257 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV6) 2 x 100 μl C258 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV7) 2 x 100 μl C259 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV8) 2 x 100 μl C260 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV9) 2 x 100 μl C261 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV10) 2 x 100 μl C262 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV11) 2 x 100 μl C491 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAVDJ) 2 x 100 μl C492 $1500.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAVDJ-8) 2 x 100 μl C493 $1500.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV1) 2 x 100 μl C453 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV2) 2 x 100 μl C454 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV3) 2 x 100 μl C455 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV4) 2 x 100 μl C456 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV5) 2 x 100 μl C457 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV6) 2 x 100 μl C458 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV7) 2 x 100 μl C459 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV8) 2 x 100 μl C460 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV9) 2 x 100 μl C461 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV10) 2 x 100 μl C462 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV11) 2 x 100 μl C463 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAVDJ) 2 x 100 μl C464 $600.00
Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAVDJ-8) 2 x 100 μl C465 $600.00
To qualify for Custom AAV Production without DNA amplification, the following amounts of the AAV vector containing the gene of interest must be supplied at >0.5μg/μl:

  • 10^9 GC/ml: 75 μg
  • 10^12 GC/ml: 250 μg
  • 10^13 GC/ml: 750 μg
For customer-supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression.

Custom AAV Production with DNA Amplification
Service NameSizeCAT. NO.PRICE
Custom AAV with DNA amplification (10^9 GC/ml) (AAV1) 5 x 200 μl C263 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV2) 5 x 200 μl C264 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV3) 5 x 200 μl C296 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV4) 5 x 200 μl C297 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV5) 5 x 200 μl C265 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV6) 5 x 200 μl C266 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV7) 5 x 200 μl C267 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV8) 5 x 200 μl C268 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV9) 5 x 200 μl C269 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV10) 5 x 200 μl C270 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAV11) 5 x 200 μl C347 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAVDJ) 5 x 200 μl C348 $600.00
Custom AAV with DNA amplification (10^9 GC/ml) (AAVDJ-8) 5 x 200 μl C349 $600.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV1) 2 x 100 μl C271 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV2) 2 x 100 μl C272 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV3) 2 x 100 μl C298 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV4) 2 x 100 μl C299 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV5) 2 x 100 μl C273 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV6) 2 x 100 μl C274 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV7) 2 x 100 μl C275 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV8) 2 x 100 μl C276 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV9) 2 x 100 μl C277 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV10) 2 x 100 μl C278 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV11) 2 x 100 μl C494 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAVDJ) 2 x 100 μl C495 $1150.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAVDJ-8) 2 x 100 μl C496 $1150.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV1) 2 x 100 μl C279 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV2) 2 x 100 μl C280 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV3) 2 x 100 μl C300 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV4) 2 x 100 μl C301 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV5) 2 x 100 μl C281 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV6) 2 x 100 μl C282 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV7) 2 x 100 μl C283 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV8) 2 x 100 μl C284 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV9) 2 x 100 μl C285 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV10) 2 x 100 μl C286 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV11) 2 x 100 μl C497 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAVDJ) 2 x 100 μl C498 $1700.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAVDJ-8) 2 x 100 μl C499 $1700.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV1) 2 x 100 μl C466 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV2) 2 x 100 μl C467 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV3) 2 x 100 μl C468 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV4) 2 x 100 μl C469 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV5) 2 x 100 μl C470 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV6) 2 x 100 μl C471 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV7) 2 x 100 μl C472 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV8) 2 x 100 μl C473 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV9) 2 x 100 μl C474 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV10) 2 x 100 μl C475 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV11) 2 x 100 μl C476 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAVDJ) 2 x 100 μl C477 $800.00
Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAVDJ-8) 2 x 100 μl C478 $800.00
*To qualify for Custom AAV Production with DNA amplification, 10μg of the AAV vector containing the gene of interest must be supplied, along with a plasmid map and full sequence for plasmid amplification. For customer supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression.

Custom siRNA AAV Production
Service NameSizeCAT. NO.PRICE
AAV siRNA Pooled Virus (AAV1)(10^9 GC/ml) 5 x 200 μl iAAV04401 $795.00
AAV siRNA Pooled Virus (AAV2)(10^9 GC/ml) 5 x 200 μl iAAV04402 $795.00
AAV siRNA Pooled Virus (AAV3)(10^9 GC/ml) 5 x 200 μl iAAV04403 $795.00
AAV siRNA Pooled Virus (AAV4)(10^9 GC/ml) 5 x 200 μl iAAV04404 $795.00
AAV siRNA Pooled Virus (AAV5)(10^9 GC/ml) 5 x 200 μl iAAV04405 $795.00
AAV siRNA Pooled Virus (AAV6)(10^9 GC/ml) 5 x 200 μl iAAV04406 $795.00
AAV siRNA Pooled Virus (AAV7)(10^9 GC/ml) 5 x 200 μl iAAV04407 $795.00
AAV siRNA Pooled Virus (AAV8)(10^9 GC/ml) 5 x 200 μl iAAV04408 $795.00
AAV siRNA Pooled Virus (AAV9)(10^9 GC/ml) 5 x 200 μl iAAV04409 $795.00
AAV siRNA Pooled Virus (AAV10)(10^9 GC/ml) 5 x 200 μl iAAV04410 $795.00
AAV siRNA Pooled Virus (AAV11)(10^9 GC/ml) 5 x 200 μl C500 $795.00
AAV siRNA Pooled Virus (AAVDJ)(10^9 GC/ml) 5 x 200 μl C501 $795.00
AAV siRNA Pooled Virus (AAVDJ-8)(10^9 GC/ml) 5 x 200 μl C502 $795.00
Custom AAV siRNA Pooled Vector 1.0 μg iAAV04400 $395.00
Titer Upgrades for Existing AAV Viruses
Service NameSizeCAT. NO.PRICE
Upgrade from 10^9 AAV Virus to High Titer AAV Virus (10^12 GC/ml) 2 x 100 μl C288 $500.00
Upgrade from 10^9 AAV Virus to High Titer AAV Virus (10^13 GC/ml) 2 x 100 μl C289 $1100.00
Upgrade from 10^9 AAV Virus to High Titer Crude Preparation AAV Virus (10^12 GC/ml) 2 x 100 μl C479 $200.00
AAV Cell Line
Service NameSizeCAT. NO.PRICE
AAV Packaging Cell Line for High Titer Virus Production 10^6 Cells C236 $250.00
Custom AAV Titration
Service NameSizeCAT. NO.PRICE
Custom AAV Titration using qPCR Assay 1 Service C238 $75.00
AAV Virus Custom Aliquoting
Service NameSizeCAT. NO.PRICE
AAV Virus Custom Aliquoting Up to 10 Vials C506 $50.00
*Minimum volume per vial = 20 μl

Additional Info

Don't Know Which Serotype To Use?

Workflow

FAQs

Is your recombinant AAV replication-deficient?
Yes, the replication and capsid genes are provided in trans when the AAV is produced, therefore the packaged virion only has the ITR sequences and the gene of interest. Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
What is the packaging capacity for AAV?
The maximum insert size is < 4.4 kb.
Is AAV stable? What is the recommended storage temperature?
It is recommended to store AAV at -80˚C for long-term storage. For short-term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
How much AAV virus should be submitted for the Custom AAV Titration service, Cat# C238?
Please send at least 50μl of virus if it is low titer. If it is expected to be high titer AAV virus, please send at least 20μl of sample.

Citations

01 Zhang, Y et al. "Hypothalamic stem cells control ageing speed partly through exosomal miRNAs.." Nature 548(7665):52-57 (2017). DOI: 10.1038/nature23282 PubMed: 28746310 Application: In vivo infection.