Custom Adeno-Associated Virus (AAV) Service
As a leading expert in AAV technology, abm offers a Custom AAV Service where we can help design, clone and/or viral package your custom AAV construct. We offer a range of AAV serotypes (1-11) as well as purifications including crude (ideal for in vitro experiments) and purified (optimal for in vivo experiments). Inquire with us below if you're looking for Custom Cloned AAV Vectors or Custom AAV Packaging.
Advantages of the AAV System:
- Used as a promising candidate for gene therapy
- Does not elicit significant immune responses in vivo
- Broad tropism - tissue specificity with different AAV serotypes
- No integration into the host genome
- Ability to transduce both proliferating and quiescent cells
- Long-term expression in non-dividing cells
Supporting Data
Left: EGFP expression (Green) in lumbar neuronal cells 4 weeks after intrathecal injection of AAV-EGFP Serotype 9 (Cat. # iAAV01509) into mice. Right: Overlay with β-tubulin (red) and DAPI (blue). Image courtesy of Dr. Douglas Lopes, King’s College London.
Service Details
Gene Synthesis and Custom AAV Cloning
| Service | Size | Cat. No. | Price |
|---|---|---|---|
| Gene Synthesis* | Per bp | C098 | Starting at USD $0.18/bp |
| Custom AAV plasmid cloning with compatible insert | 1 Service | C234 | $350.00 |
| Custom AAV plasmid cloning without compatible insert | 1 Service | C235 | $450.00 |
*Minimum charge $99.00 for Gene Synthesis. For these services, the customer must provide a gene insert under 3kb. Please contact technical@abmgood.com for more information on available cloning vectors and restriction enzyme sites to qualify for the compatible insert service.
Custom AAV Packaging Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Standard Titer Custom Recombinant AAV Packaging | 3 x 100 μl | 1011 GC/ml | Standard | 2-3 weeks | $325 | |
| High Titer Custom Recombinant AAV Packaging | 5 x 200 μl | 1012 GC/ml | Ultra-pure | 3-4 weeks | $895 | |
| Ultra-High Titer Custom Recombinant AAV Packaging | 10 x 50 μl | 1013 GC/ml | Ultra-pure | 3-4 weeks | $1695 |
Add-on Services
| Service | Cat. No. | Price |
|---|---|---|
| Plasmid Amplification for Virus Packaging Service | C314 | $100 |
To qualify for these Services 1) Customer must indicate prior to service of any expected reporter expression 2) Customer must supply their AAV vector along with plasmid map and full sequence 3) Customer must supply their AAV vector at >0.5μg/μl (10^11 GC/ml: 75µg; 10^12 GC/ml: 400µg; 10^13 GC/ml: 800µg). If the supplied amount of vector does not meet these criteria, an additional Add-on Service (Cat.No. C314) will be applied.
*Minimum volume per vial = 25 μl
Custom AAV siRNA Vector & Virus Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Standard Titer AAV siRNA Pooled Virus | 3 x 100 μl | 1011 GC/ml | Standard | 2-3 weeks | $670 | |
| Custom AAV siRNA Vector (Set of 4) | 4 x 1.0 µg | - | - | iAAV04400 | 2-3 weeks | $395 |
Custom AAV Titration
| Service | Size | Cat. No. | Price |
|---|---|---|---|
| Custom AAV Titration using qPCR Assay | 1 Service | C238 | $85.00 |
Custom AAV Aliquoting
| Service | Size | Cat. No. | Price |
|---|---|---|---|
| Custom AAV Aliquoting* | Up to 10 Vials | C506 | $5.00 |
Additional Info
Don't Know Which Serotype To Use?
Related Products
Documents
FAQs
| Is your recombinant AAV replication-deficient? |
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Yes, the replication and capsid genes are provided in trans when the AAV is produced, therefore the packaged virion only has the ITR sequences and the gene of interest. Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
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| What is the packaging capacity for AAV? |
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The maximum insert size is < 4.4 kb.
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| Is AAV stable? What is the recommended storage temperature? |
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It is recommended to store AAV at -80˚C for long-term storage. For short-term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
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| How much AAV virus should be submitted for the Custom AAV Titration service, Cat# C238? |
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Please send at least 50μl of virus if it is low titer. If it is expected to be high titer AAV virus, please send at least 20μl of sample.
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Citations
| 01 | Zhang, Y. et al. "Hypothalamic stem cells control ageing speed partly through exosomal miRNAs." Nature. 548(7665):52-57 (2017). DOI: 10.1038/nature23282. PubMed: 28746310. Application: In vivo infection. |
| 02 | Papes, F. et al. "Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content." Nature Communications. 13:2387. (2022). DOI: 10.1038/s41467-022-29942-w. PubMed: 35501322. |
| 03 | Li, J. et al. "Activation of the chemokine receptor CCR1 and preferential recruitment of Gαi suppress RSV replication: implications for developing novel RSV treatment strategies." Journal of Virology. 96(22):e0130922. (2022). DOI: 10.1128/jvi.01309-22. PubMed: 36317881 |
| 04 | Scano, M. et al. "CFTR corrector C17 is effective in muscular dystrophy, in vivo proof of concept in LGMDR3." Human Molecular Genetics. 31(4):499-509. (2022). DOI: 10.1093/hmg/ddab260. PubMed: 34505136 |