Custom Adeno-Associated Virus (AAV) Service
Recombinant Adeno-Associated Virus (AAV) is the most promising candidate for gene therapy among all viral vectors owing to its non-integrating nature and lack of pathogenicity in animal models. It is also a powerful tool in regenerative medicine due to its broad tropism and ability to transduce both proliferating and quiescent cells.
Tissue specificity of AAV can be achieved through the use of different AAV serotype capsids. abm offers packaged AAV virus as purified stock, ideal for in vivo experiments, or a crude virus preparation for in vitro cell culture experiments. Whether you are looking for over-expression or knockdown, we have it all!
Advantages of AAV:
- Does not elicit significant immune responses in vivo
- Broad tropism - tissue specificity with different AAV serotypes
- No integration into the host genome
- Ability to transduce both proliferating and quiescent cells
- Long-term expression in non-dividing cells
Our AAV infectivity Data Speaks for Itself
"If I have any special needs or concerns, they are always dealt with by the Technical Support Team. I have always been pleased with the product and will continue to use this service for my research."John Teem, Florida Department of Agriculture & Consumer Services
|Is your recombinant AAV replication-deficient?|
Yes, the replication and capsid genes are provided in trans when the AAV is produced, therefore the packaged virion only has the ITR sequences and the gene of interest. Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
|What is the packaging capacity for AAV?|
The maximum insert size is < 4.4 kb.
|Is AAV stable? What is the recommended storage temperature?|
It is recommended to store AAV at -80˚C for long-term storage. For short-term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
|How much AAV virus should be submitted for the Custom AAV Titration service, Cat# C238?|
Please send at least 50μl of virus if it is low titer. If it is expected to be high titer AAV virus, please send at least 20μl of sample.