Custom Adeno-Associated Virus (AAV) Service

As a leading expert in AAV technology, abm offers a Custom AAV Service where we can help design, clone and/or viral package your custom AAV construct. We offer a range of AAV serotypes (1-11) as well as purifications including crude (ideal for in vitro experiments) and purified (optimal for in vivo experiments). Inquire with us below if you're looking for Custom Cloned AAV Vectors or Custom AAV Packaging. 

abm also offers several ready-to-use AAV vector/virus collections:  


Advantages of the AAV System:

  • Used as a promising candidate for gene therapy
  • Does not elicit significant immune responses in vivo
  • Broad tropism - tissue specificity with different AAV serotypes
  • No integration into the host genome
  • Ability to transduce both proliferating and quiescent cells
  • Long-term expression in non-dividing cells



Our AAV infectivity Data Speaks for Itself

Workflow
Left: EGFP expression (Green) in lumbar neuronal cells 4 weeks after intrathecal injection of AAV-EGFP Serotype 9 (Cat. # iAAV01509) into mice. Right: Overlay with β-tubulin (red) and DAPI (blue). Image courtesy of Dr. Douglas Lopes, King’s College London.

"If I have any special needs or concerns, they are always dealt with by the Technical Support Team. I have always been pleased with the product and will continue to use this service for my research."

John Teem, Florida Department of Agriculture & Consumer Services

Service Details


Service Selection Assistant & Services List
How are you going to provide your insert?

What titer would you like to produce?


What serotype would you like to use?


Gene Synthesis
Service Name Size CAT. NO. PRICE
Gene Synthesis Per bp C098 Starting at USD$0.18/bp
*minimum charge $99; maximum size 3kb for AAV.
Custom AAV Plasmid Cloning
Service Name Size CAT. NO. PRICE
Custom AAV plasmid cloning with compatible insert 1 Service C234 $350.00
Custom AAV plasmid cloning without compatible insert 1 Service C235 $450.00

*For these services, the customer must provide a gene insert under 3kb. Please contact [email protected] for more information on available cloning vectors and restriction enzyme sites to qualify for the compatible insert service.

Custom AAV Production without DNA Amplification
Service Name Size CAT. NO. PRICE
Custom AAV without DNA amplification (10^11 GC/ml) (AAV1) 3 x 100 μl C239 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV2) 3 x 100 μl C240 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV3) 3 x 100 μl C290 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV4) 3 x 100 μl C291 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV5) 3 x 100 μl C241 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV6) 3 x 100 μl C242 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV7) 3 x 100 μl C243 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV8) 3 x 100 μl C244 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV9) 3 x 100 μl C245 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV10) 3 x 100 μl C246 $325.00
Custom AAV without DNA amplification (10^11 GC/ml) (AAV11) 3 x 100 μl C344 $325.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV1) 5 x 200 μl C247 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV2) 5 x 200 μl C248 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV3) 5 x 200 μl C292 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV4) 5 x 200 μl C293 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV5) 5 x 200 μl C249 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV6) 5 x 200 μl C250 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV7) 5 x 200 μl C251 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV8) 5 x 200 μl C252 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV9) 5 x 200 μl C253 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV10) 5 x 200 μl C254 $895.00
Custom AAV without DNA amplification (10^12 GC/ml) (AAV11) 5 x 200 μl C488 $895.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV1) 10 x 50 μl C255 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV2) 10 x 50 μl C256 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV3) 10 x 50 μl C294 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV4) 10 x 50 μl C295 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV5) 10 x 50 μl C257 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV6) 10 x 50 μl C258 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV7) 10 x 50 μl C259 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV8) 10 x 50 μl C260 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV9) 10 x 50 μl C261 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV10) 10 x 50 μl C262 $1695.00
Custom AAV without DNA amplification (10^13 GC/ml) (AAV11) 10 x 50 μl C491 $1695.00
To qualify for Custom AAV Production without DNA amplification, the following amounts of the AAV vector containing the gene of interest must be supplied at >0.5μg/μl:

  • 10^11 GC/ml: 75 μg
  • 10^12 GC/ml: 400 μg
  • 10^13 GC/ml: 800 μg
For customer-supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression.

Custom AAV Production with DNA Amplification
Service Name Size CAT. NO. PRICE
Custom AAV with DNA amplification (10^11 GC/ml) (AAV1) 3 x 100 μl C263 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV2) 3 x 100 μl C264 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV3) 3 x 100 μl C296 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV4) 3 x 100 μl C297 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV5) 3 x 100 μl C265 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV6) 3 x 100 μl C266 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV7) 3 x 100 μl C267 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV8) 3 x 100 μl C268 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV9) 3 x 100 μl C269 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV10) 3 x 100 μl C270 $425.00
Custom AAV with DNA amplification (10^11 GC/ml) (AAV11) 3 x 100 μl C347 $425.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV1) 5 x 200 μl C271 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV2) 5 x 200 μl C272 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV3) 5 x 200 μl C298 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV4) 5 x 200 μl C299 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV5) 5 x 200 μl C273 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV6) 5 x 200 μl C274 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV7) 5 x 200 μl C275 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV8) 5 x 200 μl C276 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV9) 5 x 200 μl C277 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV10) 5 x 200 μl C278 $995.00
Custom AAV with DNA amplification (10^12 GC/ml) (AAV11) 5 x 200 μl C494 $995.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV1) 10 x 50 μl C279 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV2) 10 x 50 μl C280 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV3) 10 x 50 μl C300 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV4) 10 x 50 μl C301 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV5) 10 x 50 μl C281 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV6) 10 x 50 μl C282 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV7) 10 x 50 μl C283 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV8) 10 x 50 μl C284 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV9) 10 x 50 μl C285 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV10) 10 x 50 μl C286 $1795.00
Custom AAV with DNA amplification (10^13 GC/ml) (AAV11) 10 x 50 μl C497 $1795.00
*To qualify for Custom AAV Production with DNA amplification, 10μg of the AAV vector containing the gene of interest must be supplied, along with a plasmid map and full sequence for plasmid amplification. For customer supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression.

Custom siRNA AAV Production
Service Name Size CAT. NO. PRICE
AAV siRNA Pooled Virus (AAV1)(10^11 GC/ml) 3 x 100 μl iAAV04401 $670.00
AAV siRNA Pooled Virus (AAV2)(10^11 GC/ml) 3 x 100 μl iAAV04402 $670.00
AAV siRNA Pooled Virus (AAV3)(10^11 GC/ml) 3 x 100 μl iAAV04403 $670.00
AAV siRNA Pooled Virus (AAV4)(10^11 GC/ml) 3 x 100 μl iAAV04404 $670.00
AAV siRNA Pooled Virus (AAV5)(10^11 GC/ml) 3 x 100 μl iAAV04405 $670.00
AAV siRNA Pooled Virus (AAV6)(10^11 GC/ml) 3 x 100 μl iAAV04406 $670.00
AAV siRNA Pooled Virus (AAV7)(10^11 GC/ml) 3 x 100 μl iAAV04407 $670.00
AAV siRNA Pooled Virus (AAV8)(10^11 GC/ml) 3 x 100 μl iAAV04408 $670.00
AAV siRNA Pooled Virus (AAV9)(10^11 GC/ml) 3 x 100 μl iAAV04409 $670.00
AAV siRNA Pooled Virus (AAV10)(10^11 GC/ml) 3 x 100 μl iAAV04410 $670.00
AAV siRNA Pooled Virus (AAV11)(10^11 GC/ml) 3 x 100 μl C500 $670.00
Custom AAV siRNA Pooled Vector 1.0 μg iAAV04400 $395.00
Custom AAV Titration
Service Name Size CAT. NO. PRICE
Custom AAV Titration using qPCR Assay 1 Service C238 $85.00
AAV Virus Custom Aliquoting
Service Name Size CAT. NO. PRICE
AAV Virus Custom Aliquoting* Up to 10 Vials C506 $50.00
*Minimum volume per vial = 20 μl

Additional Info

Don't Know Which Serotype To Use?

Workflow

FAQs

Is your recombinant AAV replication-deficient?
Yes, the replication and capsid genes are provided in trans when the AAV is produced, therefore the packaged virion only has the ITR sequences and the gene of interest. Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
What is the packaging capacity for AAV?
The maximum insert size is < 4.4 kb.
Is AAV stable? What is the recommended storage temperature?
It is recommended to store AAV at -80˚C for long-term storage. For short-term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
How much AAV virus should be submitted for the Custom AAV Titration service, Cat# C238?
Please send at least 50μl of virus if it is low titer. If it is expected to be high titer AAV virus, please send at least 20μl of sample.

Citations

01 Zhang, Y. et al. "Hypothalamic stem cells control ageing speed partly through exosomal miRNAs." Nature. 548(7665):52-57 (2017). DOI: 10.1038/nature23282. PubMed: 28746310. Application: In vivo infection. 
02 Papes, F. et al. "Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content." Nature Communications. 13:2387. (2022). DOI: 10.1038/s41467-022-29942-w. PubMed: 35501322.
03 Li, J. et al. "Activation of the chemokine receptor CCR1 and preferential recruitment of Gαi suppress RSV replication: implications for developing novel RSV treatment strategies." Journal of Virology. 96(22):e0130922. (2022). DOI: 10.1128/jvi.01309-22. PubMed: 36317881
04 Scano, M. et al. "CFTR corrector C17 is effective in muscular dystrophy, in vivo proof of concept in LGMDR3." Human Molecular Genetics. 31(4):499-509. (2022). DOI: 10.1093/hmg/ddab260. PubMed: 34505136