Custom Baculovirus Packaging Service
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To order custom services please contact us.
Custom Baculovirus Plasmid Cloning
Service Cat. No. Quant. Price
Custom Baculovirus Plasmid Cloning with Compatible Insert C173 1.0 μg $550.00
Custom Baculovirus Plasmid Cloning without Compatible Insert C174 1.0 μg $650.00
Additional Charge for Extra 1kb*** C100 N/A $100.00
**Cloning of genes over 3kb are subject to an additional charge of $100.00/kb (C100).
Please contact technical@abmgood.com for more information on available cloning vectors and restriction enzyme sites to qualify for the compatible insert service.
 
Order Custom Cloning Service
Service Description Cat. No. Quant. Price
Gene Synthesis For any sequence up to 8kb in size
Genes are fully sequenced
Must be ordered with Subcloning Service I (C158)
*minimum charge of $99.00
C098 1.0 μg $0.18/bp USD
Reporter Addition/Removal Service Addition, removal or switching of a GFP or RFP reporter in one of abm’s vectors or a vector of your choice. C129 1.0 μg $350.00
 
Custom Baculovirus Production
Service Cat. No. Quant. Price
Custom Baculovirus Production (10^6 IU/ml) C148 10 ml $750.00
Custom Baculovirus Production (10^7 IU/ml) C149 2 x 200 μl $1,550.00
 
Baculovirus Controls
Product Cat. No. Quant. Price
Baculovirus GFP Control Virus (10^6 IU/ml) BV001 10 ml $695.00
Baculovirus GFP Control Virus (10^7 IU/ml) BV002 2 x 200 μl $1,495.00
Service Details
Description
  Baculovirus is a helper independent viral system that is used to express heterologous genes. Because of their ability to efficiently infect a wide range of hosts, and their capacity to generate high yields of gene targets, the Baculovirus expression vector system (BEVS) has showed considerable promise to establish long-term gene expression in most cells. Polyhedrin and p10 are considered non-essential genes when baculovirus is in culture, allowing the coding sequences to be replaced with a sequence for the target protein. Compared to bacterial expression systems, the post-translational processing and folding of recombinant proteins produced in insect cells more closely resembles mammalian processes, and the yields of functional protein are often much greater.

Several unique features of the baculovirus system make this virus very appealing for preventive or therapeutic gene transfer. These include:
  • Generate large amounts of recombinant protein
  • Can express genes from bacteria, viruses, plants and mammals at levels from 1-500mg/l
  • Several post translational modifications possible; phosphorylation, glycosylation and acylation

By pairing this technology with our expertise in virus packaging, abm offers custom Baculovirus subcloning and virus production, allowing high level gene expression in insect cells.

An overview of abm’s custom Baculovirus service:
Step 1: Gene synthesis and/or subcloning
  • Strategy design and codon optimization
  • Gene synthesis or amplification/isolation of the gene of interest from a customer-supplied vector and subcloning it into a transfer vector with His tag
  • Sequence confirmation of insert
  • Estimated time: 2 weeks

Step 2: Virus generation and expression evaluation
  • Transformation of gene of interest into E. coli with baculoviral DNA
  • Culture of E. coli and purification using mini-prep to produce an expression bacmid (recombinant viral DNA)
  • Transfection of sf9 insect cells with expression bacmid
  • Plaque purification and viral titer assay
  • Small-scale culture to test expression level
  • Estimated time: 4-6 weeks

Optional: Protein purification
  • Virus stock preparation and amplification
  • Large-scale culture and infection of insect cells for protein expression
  • Purification from 1 litre of infected insect cell using affinity column of Ni-NTA or other column
  • Estimated time: 4-5 weeks
Product Documents
Product References
FAQs
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What advantages does a baculovirus expression system provide?
Baculovirus expression systems can be used when bacterial expression is not feasible and also when a target protein with post translational modifications is needed for functional and structural studies.
What is the biosafety level for baculovirus?
Baculovirus does not infect human cells and is considered safe to work with in BSL1.
How long does a baculovirus expression project usually take?
Most services, starting with the baculovirus expression plasmid, can be completed in 8-12 weeks.
Why choose abm’s Custom Baculovirus Production?
Capability – We can cover all aspects of your project from gene synthesis, cloning and optimization to recombinant protein production and purification.

Flexibility – Each aspect of our service is tailored to fit your specific project. We treat you as an individual, not a project number.

Value – We focus on the quality of our service, all supported by competitive pricing.

Experience – With many years of working with recombinant protein expression and virus production we can help with the most challenging of projects.

Please contact us to discuss your project and to request a personalized quotation.