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Custom CRISPR HDR Templates

ssDNA oligonucleotide
Lyophilized oligonucleotide containing your insert with flanking homology arms
Service Homology Arms + Repair Template Quantity Cat. No. Price
Custom CRISPR Repair Template (<200nt ssDNA oligonucleotide) Homology arms + insert must be less than 200nt total. Suggested homology arm size is 50nt-90nt per arm. 4 nmole C427 $275.00
 



dsDNA Vectors with Repair Template
Plasmid with Homology Arms up to <1.2kb (both left and right arms)
dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is < 1.2kb in length)
Service Homology Arms (Both Arms Combined) Repair Insert Quantity Cat No. Price
Custom CRISPR Repair Template (<1.2 kb arms, <1kb insert) < 1.2kb Custom insert <1kb 1.0 ug C428 $615
Custom CRISPR Repair Template (<1.2 kb arms, 1-2kb insert) < 1.2kb Custom insert 1-2kb 1.0 ug C429 $795
Custom CRISPR Repair Template (<1.2 kb arms, predesigned SV40-GFP-2A-Puro insert) < 1.2kb Predesigned insert with GFP and Puromycin resistance 1.0 ug C430 $875
Custom CRISPR Repair Template (<1.2 kb arms, predesigned SV40-RFP-2A-Puro insert) < 1.2kb Predesigned insert with RFP and Puromycin resistance 1.0 ug C431 $875
 
Plasmid with Homology Arms 1.2-2kb (both left and right arms)
dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is 1.2-2kb in length)
Service Homology Arms (Both Arms Combined) Repair Insert Quantity Cat No. Price
Custom CRISPR Repair Template (1.2-2kb arms, <1kb insert) 1.2-2kb Custom insert <1kb 1.0 ug C432 $855
Custom CRISPR Repair Template (1.2-2kb arms, 1-2kb insert) 1.2-2kb Custom insert 1-2kb 1.0 ug C433 $1025
Custom CRISPR Repair Template (1.2-2kb arms, predesigned SV40-GFP-2A-Puro insert) 1.2-2kb Predesigned insert with GFP and Puromycin resistance 1.0 ug C434 $1095
Custom CRISPR Repair Template (1.2-2kb arms, predesigned SV40-RFP-2A-Puro insert) 1.2-2kb Predesigned insert with RFP and Puromycin resistance 1.0 ug C435 $1095
 
Plasmid with Homology Arms >2kb (both left and right arms)
dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is > 2kb in length)
Service Homology Arms (Both Arms Combined) Repair Insert Quantity Cat No. Price
Custom CRISPR Repair Template (>2kb arms, any insert size) > 2kb Custom or predesigned insert<1kb 1.0 ug C436 Inquire
 

Service Details

Description

CRISPR knock-in experiments require the presence of an HDR template (also known as a donor plasmid), which allows for homology-directed repair (HDR) to occur after Cas9 has cut the DNA forming a double-stranded break. The CRISPR HDR template consists of an insert sequence flanked by left and right homology arms that correspond to the sequence on either side of the CRISPR-induced double stranded break. For small mutations of <50 bp, we suggest using a ssDNA oligonucleotide template, while larger insertions should be handled with one of our dsDNA donor plasmids. Choose to use our Custom CRISPR HDR templates to achieve precise genomic knock-in using the homology-directed repair pathway.

Applications

1. In-frame fusion tag
   →Tag your gene of interest with a short peptide sequence or a reporter gene
   →For this application, a ssDNA oligonucleotide repair template is recommended
2. Point mutation
   →Mutate short sequences or residues within your gene of interest
   →For this application, a ssDNA oligonucleotide repair template is recommended
3. Transgene insertion
   →Insert a larger transgene into a locus of your choice
   →For this application, a dsDNA vector repair template is recommended

Workflow

CRISPR-Custom-Repair-Template-Applications
Product Documents
FAQs
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