What is abm’s fluorophore conjugation chemistry?
abm EvaBright fluorophore dyes contain amine-reactive group (such as succinimidyl ester group) and as such, they enable covalent labelling of primary amines (lysine). Primary amines are commonly found on protein and antibodies.
Can you conjugate proteins without primary amines?
Yes, we can certainly conjugate your protein that does not contain any primary amines. We can conjugate your protein via carboxyls (-COOH), sulfhydrls (-SH) and carbonyls (-CHO) as a custom service. Please note that abm EvaBright fluorophores only react to primary amines. Simply send your inquiry with the filled custom conjugation service request form to firstname.lastname@example.org.
Can you conjugate protein or antibody from other commercial supplier?
Yes, we can. However, please note that many suppliers may provide reagents at less than optimal concentrations for conjugation and many add carriers or stabilizers (which may potentially interfere with conjugation process). In some cases, additional step may be required to purify and/or to concentrate the protein/antibody prior to conjugation process. These additional step(s) may lead to significant loss of the protein/antibody.
What are the ideal requirements for successful protein/antibody conjugation?
Protein/antibody should be purified and be above 2mg/mL or higher for efficient conjugation. The protein/antibody should be in a solution without primary amines (such as Tris buffer) or any contaminate functional groups (for example, BSA). Additional step may be required in purification, buffer exchange or contaminate removal to allow successful conjugation.
Can you conjugate protein/antibody at low concentrations?
Yes, we can conjugate your protein/antibody at low concentration, however, generally low concentration will affect the conjugation efficiency.
Can you conjugate peptide?
Yes, please provide us your peptide or alternatively, use our custom peptide synthesis option in addition to our conjugation service. Please click on the following link for our custom peptide service.
Can you conjugate other molecules, other than listed services?
We can conjugate small haptens, sugars and small molecules. Please contact our technical support specialist at email@example.com.
Can you guarantee coupling efficiency?
No, the coupling efficiency varies depending on the specific protein.
Should I expect loss of the starting material (protein/antibody)?
Yes, loss of protein/antibody may occur during the conjugation and purification process. The extent of the loss varies and depends on the protein/antibody. abm makes no guarantee on the amount of the final yield, nor the biological activity of the final product.
What will be delivered at the end of the project?
We will deliver the conjugated product in your desired buffer. We do offer additional services, including protein/antibody production and purification, please contact our technical support specialist at firstname.lastname@example.org.