Conjugation Service
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Custom Conjugation Service
With years of experience in conjugation of protein reagents and antibodies, we offer high quality, efficient and cost-saving solutions that are highly customizable to meet your assay requirements. Our protein conjugation services include fluorophore, biotin, HRP, agarose beads or magnetic beads. This service can be part of an antibody production project (i.e. Custom Mouse Monoclonal, Rabbit Monoclonal) or as a stand-alone service.

Please see our EvaBright fluorophore collection and our labelling services below.



abm’s EvaBright fluorophore advantages:
  1. Stability: chemically stable, highly active, low photobleaching and high hydrolysis resistance.
  2. pH-insensitive: Most of our EvaBright dyes are chemically stable at pH 2-11 with exceptions of EvaBright 405M SE, EvaBright 568 SE and EvaBright 620R SE that are sensitive to pH below 4.5.
  3. Water-soluble: Our EvaBright dyes are highly water-soluble (>100mg/mL). The dyes can also be dissolved in DMSO, DMF, methanol and ethanol.

Service Key Features
  • Wide selection of up to 24 unique abm fluorophores and access to our protein/antibody catalog to support your design
  • Flexible order requirement from small order to large order
  • Fast turnaround time
  • Full technical support
  • Best pricing

To order, please submit the Custom Conjugation Service Request Form with your order to quotes@abmgood.com.
Service Description Cat. No. Price
Biotinylation - Purified protein/antibody 2 mg*
- Conjugation to NHS-LC-Biotin (unless specified)
- Biotin quantitation (HABA test)
Lead time: 2 weeks
Deliverable: biotin conjugated protein/antibody
C350 $1,300.00
HRP conjugation (100µg) - Purified protein/antibody 100 µg*
- Conjugation to amine-reactive HRP
Lead time: 2 weeks
Deliverable: HRP conjugated protein/antibody
C351 $275.00
HRP conjugation (1mg) - Purified protein/antibody 1mg*
- Conjugation to amine-reactive HRP
Lead time: 2 weeks
Deliverable: HRP conjugated protein/antibody
C352 $495.00
Fluorophore conjugation (100µg) - Purified protein/antibody 100µg*
- Select from our EvaBright fluorophore collection
- Conjugation to amine-reactive fluorophore
- Purification of conjugate
Lead time: 2 weeks
Deliverable: conjugated protein/antibody
C353 $375.00
Fluorophore conjugation (1mg) - Purified protein/antibody 1mg*
- Select from our EvaBright fluorophore collection
- Conjugation to amine-reactive fluorophore
- Purification of conjugate
Lead time: 2 weeks
Deliverable: conjugated protein/antibody
C354 $695.00
Magnetic beads conjugation (100µg) - Purified protein/antibody 100µg*
- Conjugation to amine-reactive magnetic beads
- Beads specifications: superparamagnetic, 1µm, 2.0g/cm3
Lead time: 2 weeks
Deliverable: conjugated protein/antibody
C355 $375.00
Magnetic beads conjugation (1mg) - Purified protein/antibody 1mg*
- Conjugation to amine-reactive magnetic beads
- Beads specifications: superparamagnetic, 1µm, 2.0g/cm3
Lead time: 2 weeks
Deliverable: conjugated protein/antibody
C356 $1,025.00
Agarose conjugation (1mg) - Purified protein/antibody 1mg*
- Conjugation to amine-reactive agarose (NHS)
- Beads: 6% beaded agarose resin
Lead time: 2 weeks
Deliverable: conjugated protein/antibody agarose
C357 $350.00
Agarose conjugation (10mg) - Purified protein/antibody 10mg*
- Conjugation to amine-reactive agarose (NHS)
- Beads: 6% beaded agarose resin
Lead time: 2 weeks
Deliverable: conjugated protein/antibody agarose
C358 $950.00
*Customers to supply the purified protein/antibody for conjugation. Minimum quantity of protein/antibody conjugation is 0.1 mg and at a minimum concentration of 0.2 mg/mL. The protein/antibody should not contain albumin, gelatine, protease inhibitors or other amine-containing stabilizers/buffers (such as Tris). Customers can inquire additional service for purification of protein/antibody and verification of the conjugated protein/antibody via flow cytometry.

All abm’s custom conjugation service requires a non-refundable 30% deposit to initiate the project.

DISCLAIMER: Loss of protein/antibody may occur during the conjugation and purification process. The extent of the loss varies and depends on the protein/antibody. abm makes no guarantee on the amount of the final yield, nor the biological activity of the final product.
Product Documents
Product References
FAQs
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What is abm’s fluorophore conjugation chemistry?
abm EvaBright fluorophore dyes contain amine-reactive group (such as succinimidyl ester group) and as such, they enable covalent labelling of primary amines (lysine). Primary amines are commonly found on protein and antibodies.
Can you conjugate proteins without primary amines?
Yes, we can certainly conjugate your protein that does not contain any primary amines. We can conjugate your protein via carboxyls (-COOH), sulfhydrls (-SH) and carbonyls (-CHO) as a custom service. Please note that abm EvaBright fluorophores only react to primary amines. Simply send your inquiry with the filled custom conjugation service request form to quotes@abmgood.com.
Can you conjugate protein or antibody from other commercial supplier?
Yes, we can. However, please note that many suppliers may provide reagents at less than optimal concentrations for conjugation and many add carriers or stabilizers (which may potentially interfere with conjugation process). In some cases, additional step may be required to purify and/or to concentrate the protein/antibody prior to conjugation process. These additional step(s) may lead to significant loss of the protein/antibody.
What are the ideal requirements for successful protein/antibody conjugation?
Protein/antibody should be purified and be above 2mg/mL or higher for efficient conjugation. The protein/antibody should be in a solution without primary amines (such as Tris buffer) or any contaminate functional groups (for example, BSA). Additional step may be required in purification, buffer exchange or contaminate removal to allow successful conjugation.
Can you conjugate protein/antibody at low concentrations?
Yes, we can conjugate your protein/antibody at low concentration, however, generally low concentration will affect the conjugation efficiency.
Can you conjugate peptide?
Yes, please provide us your peptide or alternatively, use our custom peptide synthesis option in addition to our conjugation service. Please click on the following link for our custom peptide service.
Can you conjugate other molecules, other than listed services?
We can conjugate small haptens, sugars and small molecules. Please contact our technical support specialist at technical@abmgood.com.
Can you guarantee coupling efficiency?
No, the coupling efficiency varies depending on the specific protein.
Should I expect loss of the starting material (protein/antibody)?
Yes, loss of protein/antibody may occur during the conjugation and purification process. The extent of the loss varies and depends on the protein/antibody. abm makes no guarantee on the amount of the final yield, nor the biological activity of the final product.
What will be delivered at the end of the project?
We will deliver the conjugated product in your desired buffer. We do offer additional services, including protein/antibody production and purification, please contact our technical support specialist at technical@abmgood.com.