Custom Antibodies
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Custom Polyclonal Antibody Production
  abm offers the most compeptitive price for quality, custom, polyclonal antibody production. Our services include peptide synthesis, immunization of 2 rabbits, and peptide affinity purification of positive serum. It is all included and there is absolutely no hidden additional cost. Based on our experience, the success rate of generating a quality polyclonal antibody is over 70% with one peptide antigen and over 95% for two peptide antigen.
Service Description Cat. No. Price
Standard Service
  1. Customer provides antigens (5mg of >90% purity peptide or 5mg recombinant protein).
  2. KLH conjugation and immunization of 2 rabbits (most service providers charge an extra $100.00 for KLH conjugation).
  3. ELISA assay for titer until it reaches 1:10,000 or better.
  4. Peptide affinity purification (2-3ml purified antibody), most service providers charge an extra $500.00 for this step.
  5. The project will be finished in 12 weeks.
C090 $1350.00
Complete Service
  1. Customer provides gene name or gene/protein sequence.
  2. Peptide is designed and synthesized.
  3. KLH conjugation and immunization of 2 rabbits (most service providers charge an extra $100.00 for KLH conjugation).
  4. ELISA assay for titer until it reaches 1:10,000 or better.
  5. Peptide affinity purification (2-3ml purified antibody), most service providers charge an extra $500.00 for this step.
  6. The project will be finished in 14 weeks.
C091 $1650.00
Phospho-Peptide Service
  • Production of an antibody against a phosphorylated peptide.
C089 Inquire
Product Documents
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FAQs
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Why abm?
We work closely with you to design the process and provide regular updates on the project. You have the option to start or stop the project from any stages of the rabbit monoclonal antibody generation. Customers will be responsible for the material and labour cost incur if chose to stop the project from any stages of the development.
Can you produce an antibody against a native protein?
Yes, however, in order to guarantee the antibody is effective against the native protein, purified native protein must be used for immunization. Recombinant protein or peptide extracts can also be used to immunize, but we cannot guarantee these will be effective against the native protein due to folding and other conformational concerns. Our service guarantees that the immunized protein (native or recombinant) or peptide will be recognized by the produced antibody.
What assay do you use to confirm the functionality of the produced antibody?
For rabbit polyclonal antibody servies, we guarantee the antibodies will work against the designed peptides in ELISA format. Due to experimental variations, we do not guarantee downstream applications. It is important to understand that the customer takes full responsibility for the peptide designs.