Recombinant adenoviruses are versatile and highly efficient tools used for gene delivery and expression in mammalian cells. Numerous biological features of adenoviruses have made them the vector of choice for both in vitro and in vivo applications. They can infect a broad range of cell types with the highest efficiency. Additionally, their infection is not dependent on active host cell division. Another key feature for recombinant adenovirus is that high virus titers and high-level gene expression can be obtained in most mammalian cells.
abm produces custom recombinant adenovirus containing full-length cDNA, allowing high level gene expression in host cells or an adenovirus construct containing RNAi oligos specific to a target gene to generate efficient elimination of target gene expression.
***Please see the service agreement document for additional details and options.***
We are interested in expressing a large gene (~9000 bp). Can this gene be expressed using adenovirus and can your company clone the DNA and make the recombinant virus?
It will be challenging for gene over 8.0kb. but we will give it a try and you don't have to pay if the project fails. However, you do need to subclone into our pShuttle vector first, alternatively, we will do it for an extra charge.
How much DNA should I provide for the custom adenovirus service?
We will need the pShuttle / plasmid DNA concentration somewhere between 0.15ug/ul - 0.5ug/ul and the final amount of DNA to be at least 8ug.
Does your amplification and purification service include any additional titre verification, such as a Plaque Assay, RCA Assay or TCID50 Assay?
The viral titre will be calculated by end-point dilution assay. Any additional assays required (such as those listed above) can be provided on request, at an additional cost.
Can I provide crude 293 lysate of adenovirus stock to be amplified and purified?
Yes, we will require 5ml of crude 293 lysate to perform the amplification service.
How much adenovirus stock do I need to ship for the amplification service?
We require 250ul of adenovirus stock, shipped on dry ice.
Do you sell the adenoviral vectors that you have used to make any of your prepackaged adenovirus?
Our adenoviral vectors are currently only available as packaged viral particles. We do offer custom cloning services if further modifications are desirable.
What will be the final composition of your high titer, purified adenovirus?
Our high titer adenoviruses are re-suspended in 50mM Tris-HCl (pH7.0) with 5% glycerol