Custom 5’ UTR Reporter Vector

Every gene has a 5’ UTR and 3’ UTR (Untranslated Region) upstream and downstream, respectively, of the coding region. While 3’ UTRs are the major focus of regulatory research, 5’ UTRs have also been found to mediate translational regulation via elements such as upstream start codons (uAUGs), upstream open reading frames (uORFs), secondary structures, RNA binding protein (RBP) binding motifs, and miRNA binding.

abm’s 5’ UTR Reporter Vectors consist of the 5’ UTR of the gene of your choice preceding a GFP or Luciferase reporter, driven by a β-actin promoter. These vectors are ideal for studying the regulation of protein expression by 5’ UTR elements. The 5’ UTR reporter constructs can be combined with our Site-Directed Mutagenesis Service (Cat. No. C047) in order to define RBP or miRNA binding sites, or investigate the effects of uAUGs or uORFs on gene expression.




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Service Details

PRODUCTREPORTERAMOUNTCat. No.PRICE
Custom 5' UTR Reporter Lentiviral Vector Subcloning (GFP) EGFP 1.0 µg C451 $350.00
Custom 5' UTR Reporter Lentiviral Vector Subcloning (Luc) Firefly Luciferase 1.0 µg C452 $350.00
CONTROLSREPORTERAMOUNTCat. No.PRICE
Blank 5' UTR Reporter Lentiviral Vector (GFP) EGFP 1.0 µg LV657 $150.00
Blank 5' UTR Reporter Lentiviral Vector (Luc) Firefly Luciferase 1.0 µg LV658 $150.00
ADD-ONSAMOUNTCat. No.PRICE
Site Directed Mutagenesis Service Single Clone C047 $150.00
Plasmid Amplification (10-20 µg) 10‑20 µg C309 $75.00
Plasmid Amplification (80-100 µg) 80‑100 µg C310 $150.00
Bacterial Glycerol Stock 1 Clone C139 $25.00
Custom Lentivirus Packaging Service 106‑1010 IU/ml Click here From $650.00



All of abm’s UTR reporter vectors are available as ready-to-use DNA plasmids, or they can be packaged into lentiviral particles. Our UTR reporter vectors can be directly transfected into target cells for transient expression, while lentiviral particles allow difficult-to-transfect cells, such as primary cells, to be efficiently transduced for stable, long-term expression.

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Citations

01 Xu, Y et al. "Bile acid and cigarette smoke enhance the aggressive phenotype of esophageal adenocarcinoma cells by downregulation of the mitochondrial uncoupling protein-2." Oncotarget 8.60:101057–101071. (2017). DOI: 10.18632/oncotarget.22380 Application: Lentiviral transfection.