Dual Luciferase Reporter Lentivectors
abm’s Dual Luciferase Reporter system makes it easy to normalize your luciferase data. Our system is designed such that one luciferase responds to 3'UTR-dependent, 5'UTR-dependent , or promoter-dependent expression, while a second luciferase is constitutively expressed from the same plasmid for uniform activity.
Never worry about the luciferase activity being skewed across test conditions and replicates by differences in the number of cells and the number of plasmid copies per cell. Simply divide the P. pyralis Luc activity by the R. reniformis Luc activity to normalize the measured effect on the 3'UTR/ 5’UTR/promoter.
Looking for a single Luc system? Inquire with us today!
|Dual Luciferase 3'UTR Reporter lentivector||Dual Luciferase Promoter Reporter lentivector||Dual Luciferase 5'UTR Reporter lentivector|
"If I have any special needs or concerns, they are always dealt with by the Technical Support Team. I have always been pleased with the product and will continue to use this service for my research."John Teem, Florida Department of Agriculture & Consumer Services
|SERVICE||UNIT||Cat. No.||UNIT PRICE|
|Gene Synthesis||Per bp||C098||from USD $0.18/bp|
|Vector Backbone for Cloning||Not a Deliverable||C318||$350.00|
|Subcloning Service I||1.0 µg||C096||$125.00|
|Upgrade UTR Library to Dual-Luciferase Reporter||1 Construct||C523||$195.00|
|SERVICE||UNIT||CAT. NO.||UNIT PRICE|
|pLenti-UTR-Dual-Luciferase Blank Vector||1.0 µg||m031||$165.00|
|Lenti-UTR-Dual-Luciferase Blank Virus||106 IU/ml, 3.0 ml||m032||$625.00|
|Blank UTR (Control) Dual Luciferase Stable Cell Line||106 cells/ml, 1.0 ml||m033||$1350.00|
|Blank Promoter Reporter Lentiviral Vector (Dual Luc)||1.0 µg||m034||$165.00|
|CMV Promoter Reporter Lentiviral Vector (Dual Luc)||1.0 µg||m035||$165.00|
|pLenti-5'UTR-Dual-Luciferase Blank Vector||1.0 µg||m036||$165.00|
Additional InfoDual Luciferase 3'UTR Reporter system:
Dual Luciferase 5'UTR Reporter system:
Dual Luciferase Promoter Reporter system:
|For the Antibiotic Switching Service, can I change the gene to a resistance marker other than puromycin or neomycin?|
Yes, we also offer hygromycin and blasticidin. You can also send us a plasmid containing a marker to be subcloned, or tell us the sequence that you would like us to synthesize. Please email [email protected] for further information.
|01||Mazan-Mamczarz, K et al. "Down-Regulation of eIF4GII by miR-520c-3p Represses Diffuse Large B Cell Lymphoma Development ." PLOS GENETICS 10 (1):e1004105 (2014). DOI: 10.1371/journal.pgen.1004105PubMed: 24497838 Application: 3'UTR Lenti-reporter-Luciferase vector|
|02||Xu, Y et al. "Bile acid and cigarette smoke enhance the aggressive phenotype of esophageal adenocarcinoma cells by downregulation of the mitochondrial uncoupling protein-2." Oncotarget 8.60:101057–101071 (2017). DOI: 10.18632/oncotarget.22380 Application: Lentiviral transfection.|
|02||Pacifico, F. et al. "The chemokine scavenging receptor D6/ACKR2 is a target of miR-146a in thyroid cancer." Genes & cancer 5-6:577 (2017). PubMed: 10.18632/genesandcancer.141 Application: General lentivirus.|