Dual Luciferase Reporter Lentivector Service
abm’s Dual Luciferase Reporter system allows for easy normalization of your luciferase data - never worry about the luciferase activity being skewed across test conditions or replicates by differences in the number of cells or plasmid copy number per cell.
Our system is designed such that Firefly Luciferase (P. pyralis) responds to a 3'UTR-dependent, 5'UTR-dependent, or promoter-dependent expression, while a Renilla Luciferase (R. reniformis) is constitutively expressed from the same plasmid for uniform activity. Simply divide the Firefly Luciferase activity by the Renilla Luciferase activity to normalize the measured effect on the 3'UTR/5’UTR/promoter.
Check out our Site-Directed Mutagenesis Service to further characterize your 5' UTR/3'UTR/promoter. We can mutate miRNA binding sites, transcription factor binding sites and deliver both wild type and mutated sequences in our Dual Luciferase systems.
Additional Resources:
"If I have any special needs or concerns, they are always dealt with by the Technical Support Team. I have always been pleased with the product and will continue to use this service for my research."
John Teem, Florida Department of Agriculture & Consumer ServicesService Details
Core Services
| SERVICE | UNIT | Cat. No. | UNIT PRICE |
|---|---|---|---|
| Gene Synthesis | Per bp | C098 | from USD $0.18/bp |
| Vector Backbone for Cloning | Not a Deliverable | C318 | $350.00 |
| Subcloning Service I | 1.0 µg | C096 | $125.00 |
| Upgrade UTR Library to Dual-Luciferase Reporter | 1 Construct | C523 | $195.00 |
Controls
| PRODUCT | UNIT | Cat. No. | UNIT PRICE |
|---|---|---|---|
| UTR-Dual-Luciferase Control Lentivirus | 107 IU/ml, 4 x 500 µl | m032 | $195.00 |
| Dual Luciferase Control Stable Cell Line | 106 cells/ml, 1.0 ml | m033 | $1350.00 |
| pLenti-Promoterless-Dual-Luciferase Control Vector | 1.0 µg | m034 | $165.00 |
| pLenti-CMV-3'UTR-Dual-Luciferase Control Vector | 1.0 µg | m035 | $165.00 |
| pLenti-Beta actin-5'UTR-Dual-Luciferase Control Vector | 1.0 µg | m036 | $165.00 |
Additional services such as Site-Directed Mutagenesis, reporter switching, and high-titer lentiviral packaging are available upon request.
Additional Info
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| Dual Luciferase 3'UTR Reporter Lentivector | Dual Luciferase Promoterless Reporter Lentivector | Dual Luciferase 5'UTR Reporter Lentivector |
Dual Luciferase 3'UTR Reporter system:
Interference with a 3'UTR (e.g. by miRNA) destabilizes the P. pyralis luciferase mRNA, and this effect is measurable with a luciferase assay. Divide the activity of P. pyralis luciferase by the activity of the constitutively expressed R. reniformis luciferase for normalization. Thousands of human, mouse, and rat 3'UTR sequences are also available in our 3' UTR miRNA Library for an upgrade (Cat. No. C523) to our Dual Luciferase system.
Dual Luciferase Promoterless Reporter system:
Close to 18,000 human and 17,500 mouse promoters are eligible for abm's custom promoter reporter lentiviral vector services. In our Dual Luciferase system, your promoter's activity in different cell types or in the presence of different transcription factors determines the activity of P. pyralis luciferase. To normalize it, simply divide it by the activity of R. reniformis luciferase that is constitutively expressed from the SV40 promoter. Studying a specific type of tissue? Please inquire with us for tissue-specific promoters!
Dual Luciferase 5'UTR Reporter system:
Transcribed from the β-actin promoter, the 5'UTR determines the activity of P. pyralis luciferase. Simply divide it by the activity of R. reniformis luciferase that is constitutively expressed in our Dual Luc system for normalization.
Related Products
FAQs
| For the Antibiotic Switching Service, can I change the gene to a resistance marker other than puromycin or neomycin? |
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Yes, we also offer hygromycin and blasticidin. You can also send us a plasmid containing a marker to be subcloned, or tell us the sequence that you would like us to synthesize. Please email [email protected] for further information.
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Citations
| 01 | Mazan-Mamczarz, K et al. "Down-Regulation of eIF4GII by miR-520c-3p Represses Diffuse Large B Cell Lymphoma Development ." PLOS Genetics. (2014) 10(1):e1004105. DOI: 10.1371/journal.pgen.1004105PubMed: 24497838 |
| 02 | Xu, Y et al. "Bile acid and cigarette smoke enhance the aggressive phenotype of esophageal adenocarcinoma cells by downregulation of the mitochondrial uncoupling protein-2." Oncotarget. (2017) 8.60:101057–101071. DOI: 10.18632/oncotarget.22380 |
| 03 | Pacifico, F. et al. "The chemokine scavenging receptor D6/ACKR2 is a target of miR-146a in thyroid cancer." Genes & Cancer. (2017) 5-6:577. DOI: 10.18632/genesandcancer.141 |
| 04 | Ma, L et al. "Electroacupuncture-Regulated miR-34a-3p/PDCD6 Axis Promotes Post-Spinal Cord Injury Recovery in Both In Vitro and In Vivo Settings." Journal of Immunology Research. (2022) 1–17. DOI: 10.1155/2022/9329494 |
| 05 | Outeiro-Pinho, G et al. "Epigenetically-regulated miR-30a/c-5p directly target TWF1 and hamper ccRCC cell aggressiveness." Translational Research. (2022) 249, 110-127. DOI: 10.1016/j.trsl.2022.06.009 |
