ELISA Ultrasensitive TMB Substrate for HRP
Cat. No.
G449
Unit
1.0 L
Price
$375.00
| Cat. No. | G449 |
| Name | ELISA Ultrasensitive TMB Substrate for HRP |
| Category | Gels, Blots & ELISAs |
| Description |
3,3'5,5'-Tetramethylbenzidine (TMB) is a safe-sensitive substrate for the detection of horseradish peroxidase (HRP) activity. It yields a blue colour (Amax = 652nm) and changes to yellow (Amax = 450nm) upon the addition of either sulfuric or phosphoric acid. ABM’s ELISA Ultrasensitive TMB Substrate for HRP yields the greatest sensitivity among the TMB substrates. |
| Application |
Suitable for kinetic and endpoint assays involving Horseradish Peroxidase (HRP). |
| Recommended Protocol | 1. Complete all required incubations with antibodies, probes and HRP labeled reagents. 2. Wash plate, at least 4 times, with phosphate buffered saline or Tris buffered saline containing 0.1% Tween-20. 3. After the final wash, shake and blot all residual buffers from plate wells. 4. Add 0.1ml of ABM’s Ultrasensitive TMB Substrate for HRP to appropriate wells and incubate 5-30 minutes. 5. The reaction can be monitored as a function of time for kinetic assays or stopped with 0.1ml of 0.3mol/l of sulphuric acid or 0.1% sodium fluoride and read at 650nm. 6. If reaction needs to be converted to yellow diimine, add 0.1ml of either sulfuric or phosphoric acid and record the absorbance within 5 minutes. |
| Storage Condition |
Store at room temperature (15-28°C) or at 2-4°C and then warm up to assay temperature prior to use. Prevent exposure to direct sunlight. Discard if solution turns blue or turbid. |
| Guarantee |
abm guarantees that all our Tissue Slides will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
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