Full Length Plasmid Sequencing Service
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Applied Biological Materials, Inc. is an Illumina Certified Service Provider, dedicated to ensuring the delivery of the highest-quality data available for genetic analysis applications. Click here for more details about the Illumina CSpro program.
  Plasmid Verification Services at your fingertips.

Prices are in US dollars. Please contact technical@abmgood.com if you need a quotation in Canadian dollars. Prices include sample QC, library preparation and quantification, and sequencing. Custom sequencing requirements can be quoted by contacting our technical support team.
Service/Description Cat. No. Min. Samples Price*
Plasmid Sequencing (Paired-End)
Full Length Plasmid Sequencing (100Mb, 2x75bp PE) IW10001 1$165.00*
 
Additional Sequencing Services
End-sequence validation (Sanger sequencing) IW10002 1$30.00*
 
Bioinformatics Analysis
Raw data provided in FASTQ format if customer prefers to assemble themselves.
de novo Genome Assembly & Plasmid Annotation
- de novo Assembly (<50kb Plasmid, >100X coverage)
- Snapgene and PlasMapper Plasmid Annotation
Included
Other bioinformatics analysis available, please contact us for a quote.
 
Data Storage Service
Data Storage (per additional 3 months) IS10001 1$150.00
*Price per Sample
Service Details
Description of Plasmid-Seq Service
    abm’s Plasmid Sequencing service utilizes the power of next generation sequencing for plasmid verification (obtaining the full length sequence of any plasmid). Unexpected mutations and wrong sequence information can affect downstream applications and lead to unnecessary troubleshooting slowing down your research progress. With as little as 1 ng of plasmid DNA, the plasmid sequencing service can provide comprehensive full length coverage for any plasmid, something that has only recently become achievable. A maximum plasmid size of 50kb is included with this service; larger plasmids can be accommodated for an additional fee. Validation of the ends of the contig is highly recommended, as NGS usually does not produce a perfect assembly towards the beginning and ends of the contig, an issue that is easily resolved with Sanger sequencing and appropriate primer design.

abm’s Plasmid-seq service overview:


Key features for abm's Plasmid-Seq service:
  • high quality, long contig plasmid assembly sequences from outstanding raw read accuracy and paired end assembly
  • full length sequencing of unknown plasmid or verification of known plasmid (reference sequence required)
  • Sanger end sequence validation (highly recommended) offered as an additional service
  • diagnostic restriction digest tests and agarose gel QC confirm assembly size
  • identification of restriction enzyme cut sites, ORFs, and other potentially useful features

A standard PDF summary report highlighting data statistics and results in industry standard format are provided with each project. Raw fastq data and de novo assembly results in fasta format are also released. Raw data will be stored for 3 months. All of abm's sequencing services are performed by a group of specially trained and experienced scientists partaking in a streamlined workflow.
Sample Requirement Guidelines Click To Open
    Our technical support team will be happy to work on confirming the details of your NGS service. Please contact our specialists at technical@abmgood.com to get your order confirmation number. Any samples shipped to our facility without an order confirmation number in the attention line will be destroyed and disposed immediately upon receipt.
Sample Submission Form for Plasmid-Seq Click To Open
Sample Shipping Instructions:
  • Shipping DNA for NGS to abm Click To Open
  • Confidentiality of Plasmid Sequencing Results
        All customer information is held in strict confidence. All materials and information sent to us and the products produced by us for the order are the property of the customer and will be returned to the customer or discarded in a confidential manner. We only archive customer materials when instructed to.
    Project Design/Advice
        Please email us at NGS@abmgood.com for assistance with starting your Plasmid-Seq project or any inquiries about our next generation sequencing services.
    Product Documents
    Product References
    FAQs
    Submit a new question:
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    Is your company certified as a service provider for Illumina sequencing?
    Yes, we are a certified Illumina service provider.
    What kind of analysis software do you use for sequence analysis?
    The Illumina sequencers come with the integrated software that can perform the regular bioinformatics analysis functions. In addition, we have extensive experience with other open access software such as Galaxy, Adnuril, BioBike, etc. In other words, we are willing to perform additional bioinformatics analysis with different software and tools to satisfy a variety of demands from our customers.
    What kind of QC methods do you adopt for the customer's sample(s)?
    We will perform QC on your Total RNA samples prior to sequencing them, using the Agilent Bioanalyzer (industry standard) to determine the RNA Integrity Number (RIN). If the RIN is lower than 8, we consider these samples as having failed QC. If the samples do not pass QC, we will ask you to send us new samples. If we do not receive new samples, thereby cancelling the sequencing order, there will be a QC fee of $50/sample. This fee will be waived if you choose to continue with the sequencing order. A strict positive and negative control will be applied to this QC process.

    The library QC will also be performed using the Agilent Bioanalyzer to determine library size and purity. Also, prior to loading the libraries on the sequencer, we perform qPCR quantification. The cost for this is included in the sequencing service.

    For the QC of the final data from our sequencing service, the integrated software will generally do the job and provide adequate QC information, however, we can also provide additional QC data such as FASTQC upon request.

    The data we output will pass our Q30 filter, which means that the error rate in base calls is less than 1 in 1000, or 0.1%. We will get a percentage at the end of the run which states the percentage of bases that have a Q score > 30, and this percentage is usually ≥85%.
    What is the lead time for your WGS, WES, and RNA-Seq services?
    Our sequencing lead times depend on workload, but are typically 2-4 weeks for sequencing data + 2-3 weeks for standard analysis. Please inquire for lead times for more detailed analysis.
    Can you recommend any preferred RNA/DNA isolation kits to provide the best quality samples for these services?
    For miRNA sequencing, you may isolate your RNA sample with a mirVana™ miRNA Isolation Kit,
    mirVana™ PARIS™ Kit or PureLink® miRNA Isolation kit to enable adequate enrichment of your sample. If preferred, it should be okay to use Qiagen's kit, as long as the RNA Integrity Number (RIN) of the sample is above 8.0 to make sure there is no significant degradation. (Samples with a RIN lower than 8.0 may contain smaller degraded RNA fragments that can be sequenced in addition to the miRNAs). We prefer receiving Total RNA sample however so we can perform the bioanalyzer QC to check the RNA quality before beginning library construction. There will be no extra-charges if you submit Total RNA instead of isolated miRNA.

    For miRNA sequencing we require 200ng-2ug of total RNA in 10ul of nuclease free water, as quantified with a fluorometric method. Lower amounts might result in inefficient ligation and low yield. If you are supplying purified miRNA, please submit a minimum of 50-100 ng of purified small RNA in 10ul is required. Purified small RNAs must be in nuclease free water or 10 mM Tris-HCI, pH 8.5.

    For other services, there are generally no preferred DNA/RNA isolation kits as long as minimum requirements for QC are met.

    Do I need any special software to view the data report files?
    You will first need to unzip the fastq.gz files with winrar to view with vim, nano, gedit, Notepad plus plus. Nextgen workbench generally can view the data immediately without unzipping.

    The RNASeq_sample_cufflinks_output.tar.gz will also need to be unzipped to see the text files by vim, nano, gedit, or Notepad plus plus. The unzipped data an also be imported as text into the data tab of Excel 2007. Other versions may differ.

    To analyze further, Excel can sort, filter, find, match, etc and has many functions for conditional coloring, graphing, etc.
    For the data, why are there no "ALT", "POS" and "ID" in your CSV file?
    CSV files are meant to be used in conjunction with the raw fastq/bam files by certain commercial bioinformatics software. Many have a proprietary algorithms that takes the ALT POS and ID from the Fastq and indexes them for its own use. They are not meant to be used alone.
    Would you let me know the description for the following: Genotype Sample_0, Coverage Sample_0, Coverage By Allele Sample_0, Variant Quality Sample_0, and Genotype Quality Sample_0?
    Genotype (Sample Name) = nucleotide. Coverage (Sample Name) = will fail if there are insufficient reads. Coverage by allele (Sample Name) = will fail on one strand (+/-) read if only one side of the pair was sequenced. Variant Quality (Sample Name) = confidence of variant called. Genotype Quality (Sample Name) = confidence of genotype sequenced.
    Concerning the column of "CHROM", would you let me know the rules of naming?
    type of accession number| actual number| type of sequence| actual curated version used
    Does your NGS service including RNA isolation?
    We do not provide RNA isolation service by default. However we can provide this service. For more information, please refer to Cat#IR19001.
    How many biological replicates do I need for each condition?
    To increase confidence and reduce experimental error it is suggested that you submit at least 3 replicates per sample. Note that this is to serve as a guideline only and the final number of replicates and samples is to be determined by the end user based on their final experimental conditions.