Mouse Antibody Isotyping Kit
Cat. No.
G617
Unit
20 Tests
Price
$415.00
Specifications
| Cat. No. | G617 |
| Name | Mouse Antibody Isotyping Kit |
| Category | Antibody Isotyping |
| Description |
The Mouse Antibody Isotyping Kit offers ELISA-grade sensitivity in determining monoclonal antibody classes (IgG1, IgG2a, IgG2b, and IgM) and subclasses (ĸ and λ light chains) within 5 minutes. Knowing the monoclonal antibody isotypes and subclasses is useful in choosing the most appropriate immunoglobulin purification method. This kit can be used to screen hybridoma clones for desirable isotype and/or subclass. The ideal sample type is hybridoma culture supernatant or purified monoclonal antibody samples; however ascites fluid samples can also be used. Add your diluted sample to the loading well and specific class- and subclass-soluble complexes will form with antibodies in the sample that travels through the membrane, and are resolved on anti-isotype and class-specific antibody-impregnated membrane to display a red band. |
| Product Features | Applications/Features • Simple to use with colour-readout results in 5 minutes • Rapid and reliable results similar to standard ELISA-based isotyping assays • Able to detect antibodies in cell culture supernatant and ascites fluid samples at ≥ 100 ng/mL • Specific assay with no cross-reactivity with fetal bovine serum (FBS) or among each isotype • Red control line appears to indicate a successful run |
| Storage Condition | This product is stable at cool, dry, room temperature. For extended storage, keep at 4°C. The expiration date is one (1) year from the date of receipt. |
| Guarantee | abm guarantees that all our Tissue Slides will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
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