- efficient and convenient -- all you need to do is to send in a cell culture sample.
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- convenient -- we eliminate the need for you to acquire special supplies and equipment needed to properly decontaminate cell culture samples.
Note: Our mycoplasma detection method is qualitative and thus, detection limit refers to presence or absence of PCR product as seen on an agarose gel. If mycoplasma is absent, no amplified PCR product should be seen. A 15% non-refundable fee is required to initiate the service.
For frozen cells, please ship two vials (at least 10^6 each) on dry ice. Another method is to send us two T25 flask of live cells per sample, at 50-60% confluency. The flask should be filled with complete medium without any air bubble and at room temperature. The flask can be filled with medium using a 50ml culture tube.
For cell culture medium, please ship at least 0.5ml of medium with ice packs. Please ensure that the samples have been in culture with the cells for at least 48-72 hours.
For frozen cells, please ship at least two vials (at least 10^6 each) on dry ice. Another method is to send us two T25 flask of live cells per sample, at 50-60% confluency. The flask should be filled with complete medium without any air bubble and at room temperature. The flask can be filled with medium using a 50ml culture tube.
If your cells require medium other than DMEM and RPMI, we will need 1 L of medium and any applicable growth factors from you for the project. Also, please provide coated 6-well plates and T25 flasks if the cells require specially treated culture vessels.
Even though our mycoplasma treatment has shown a very high success rate but so far no antibiotic has shown permanent removal of all mycoplasma as some species may become resistant to the treatment. We guarantee the absence of mycoplasma in treated samples within our detection limit using PCR based detection method.
In such cases of extensive mycoplasma contamination, we recommend to use anti-mycoplasma reagents for prevention if you cannot replace the cells and constant monitoring of mycoplasma levels. There are some genetic approaches reported in literature that can be tried in such cases.