Mycoplasma Detection & Decontamination Service

Contamination of cell cultures with Mycoplasma is a widespread laboratory problem that can jeopardize important experimental results. Simply submit your cell culture samples and we will analyze them for Mycoplasma contamination and perform Mycoplasma decontamination (if applicable).



Service Details

Service NameunitCAT. NO.unit price
Mycoplasma Spp. Detection 1 Sample C214 $52.00
Mycoplasma Spp. decontamination 1 Sample C232 $1500.00



Additional Info

Mycoplasma Detection:

Cell culture samples sent in by the customer will be analyzed for Mycoplasma contamination and a report will be provided within 10 business days of receiving the cells. Our PCR-based Mycoplasma detection method will detect over 200 strains of Mycoplasma.

  1. Excellent Results: Our Mycoplasma detection technique is specially designed for sensitivity and accuracy.
  2. Efficient & Convenient : Simply send in a cell culture sample: serum/supernatant, frozen cells, or live cells.

Mycoplasma Decontamination:

Mycoplasma-contaminated cell cultures sent in by the customer will be treated with anti-Mycoplasma cocktails and delivered back to the customer once decontamination has been confirmed. A report will be provided with the cell culture to certify the success of the treatment. The entire process will be completed within approximately 45 business days of receiving the cell culture. You will receive two vials that are Mycoplasma absent, as tested by our PCR-based method for Mycoplasma detection. For additional vials, please inquire.

  1. Effective: Removal of Mycoplasma from cell culture samples based on PCR test detection limit.
  2. Convenient: We take care of the complicated process of decontaminating your cell culture samples for you.
  3. Supportive: We will retain 1 vial of decontaminated cells for up to 30 days upon order completion should a back-up vial be needed (e.g. shipment issues etc.)

How to Submit Samples:

 

Serum/Supernatant Please ship at least 0.5 ml of serum media or cell culture supernatant with ice packs. Please ensure that the samples have been in culture with the cells for at least 48-72 hours.
Frozen Cells Please ship two vials (at least 106 cells each) on dry ice and supply all the components required to make at least 1 L of complete medium (base medium, growth factors, serum, supplements etc.), and a complete cell culture protocol. Please provide coated 6-well plates and T25 flasks if the cells require specially-treated culture vessels. An additional 1 week lead time is required if frozen cells are supplied.
Live Cells Two T25 flasks of live cells per sample, at 50-60% confluency. The flasks should be filled with complete medium without any air bubble and at room temperature. The flasks can be filled with medium using a 50 ml culture tube. For the Decontamination Service, please also supply all the components required to make at least 1 L of complete medium (base medium, growth factors, serum, supplements etc.), and a complete cell culture protocol. Please provide coated 6-well plates and T25 flasks if the cells require specially-treated culture vessels.

FAQs

How many vials of cells do we get after the Mycoplasma Decontamination Service?
You will receive two vials that are Mycoplasma-absent, as tested by our standard PCR-based method for Mycoplasma detection. Additional vials will cost extra. Note: Our Mycoplasma detection method is qualitative and thus, detection limit refers to presence or absence of PCR product as seen on an agarose gel. If Mycoplasma is absent, no amplified PCR product should be seen.
How do you confirm the sample is Mycoplasma-free?
We use a PCR method to test for the presence of Mycoplasma (Cat. No. G238). This kit detects over 200 species of Mycoplasma and we do consecutive testings to make sure that the Mycoplasma does not return after treatment stops. We can also have a third party certify that the cells are free of Mycoplasma, at an additional charge.
Can I buy a detection kit and test my own samples first before submitting them for elimination?
Certainly. The link to the detection kit is http://www.abmgood.com/PCR-Mycoplasma-Detection-Kit-G238.html. This kit can detect over 200 different types of Mycoplasma species.
What is the method used to remove Mycoplasma?
abm has several methods of treating Mycoplasma-contaminated samples (e.g. potent antibiotics cocktail or non-antibiotics-based treatment). Our methods show no toxicity to most cells, as determined by gene array assay.
If the Mycoplasma infection keeps coming back after we received treated cells, what guarantees are provided?
We monitor the cells for 2 weeks after we stop treatment to ensure that the Mycoplasma contamination does not re-occur. Even though our Mycoplasma treatment has shown a very high success rate, so far no antibiotic can effect permanent removal of all Mycoplasma, as some species may become resistant to the treatment. We guarantee the absence of Mycoplasma in treated samples within our detection limit using a PCR-based detection method. In such cases of extensive Mycoplasma contamination, we recommend to use anti-Mycoplasma reagents for prevention if you cannot replace the cells, and constant monitoring of Mycoplasma levels. There are some genetic approaches reported in literature that can be tried in such cases.
Can you provide additional vials for Mycoplasma Decontamination Service?
Yes, we can offer Additional Vials of Delivered Cells (Cat. No. C144).

Citations

01 Schommer, J. et al. "27-Hydroxycholesterol increases α-synuclein protein levels through proteasomal inhibition in human dopaminergic neurons." BMC Neurosci 19:17 (2018).DOI: 10.1186/s12868-018-0420-5
02 Sanlorenzo, M. et al. "BRAF and MEK inhibitors increase PD1-positive melanoma cells leading to a potential lymphocyte-independent synergism with anti-PD1 antibody." Clin Cancer Res. 17:1914 (2018).DOI: 10.1158/1078-0432.CCR-17-1914
03 Xu, A. et al. "Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing." Stem Cell Res. 29:215-219 (2018). DOI: 10.1016/j.scr.2018.04.013
04 Jagadish, N. et al. "Heat shock protein 70–2 (HSP70-2) is a novel therapeutic target for colorectal cancer and is associated with tumor growth." BMC Cancer 16:561 (2016).DOI: 10.1186/s12885-016-2592-7
05 Jagadish, N. et al. "A-kinase anchor protein 4 (AKAP4) a promising therapeutic target of colorectal cancer." Journal of Experimental & Clinical Cancer Research 34:142 (2015).DOI: 10.1186/s13046-015-0258-y
06 Black, L.A. et al. "In vitro activity of chloramphenicol, florfenicol and enrofloxacin against Chlamydia pecorum isolated from koalas (Phascolarctos cinereus)." Aust Vet J. 11:420-423 (2015).DOI: 10.1111/avj.12364