Next Generation CRISPR Validation Service

abm is offering CRISPR-Cas9 knockout validation through Next-Generation Sequencing. Through targeted amplicon sequencing of the Cas9 target site, abm can harness the power of high throughput sequencing to quantify the amount of knockout allele expressed versus the wild-type. Stop guessing and know with clarity of your edited genome.




abm Next Generation CRISPR Validation Service

illumina certified service provider

abm is an Illumina Certified Service Provider, dedicated to ensuring the delivery of the highest-quality data available for genetic analysis applications.

"I am a fan of abm: great product quality, great price, great rewards and great experience."

Dr. Vincent Emond, Centre de recherche du CHU de Québec CHUL

Service Details

Core Services
Paired-end sequencingUNITCAT. NO.Unit Price
CRISPR-Cas9 Targeted Amplicon-Seq 1 Sample IA00100 $645.00
Targeted Amplicon-Seq for Additional Samples 1 Sample IA00200 $220.00
Bioinformatics Services
SERVICE NAMEUNITCAT. NO.UNIT PRICE
Reference Genome Import (<10Mb genome) 1 Genome IB00001 $55.00
Reference Genome Import (>10Mb genome) 1 Genome IB00002 $220.00
Additional Services
SERVICE NAMEUNITCAT. NO.UNIT PRICE
DNA Isolation for NGS 1 Sample IW19001 $55.00
Sample and Library QC 1 Library IR18001 $55.00
Data Storage (per additional 3 months) IS10001 $150.00
Hard Drive of NGS Results - Up to 2 Tb 1 Hard Drive IS10003 $125.00

Key Features for abm's Next Generation CRISPR Validation Service

Sequencing PlatformIllumina
Starting Material Minimum 100ng of gDNA
Sequencing Scale 100K reads
Sequencing Configuration 2 x 75bp PE
2 x 150bp PE
2 x 250bp PE
Service Includes Primer design and synthesis
Targeted amplification
Library preparation
Sequencing
Data anlaysis
Bioinformatics Analyses Read alignment
Variation detection
Turn-around Time About 4-6 weeks from passing sample QC
Data Storage 3 months
abm’s Next Generation CRISPR Validation service is a complete service from primer design to amplicon sequencing and we can handle amplicons of any sizes. Our service is also flexible for you to submit your own amplicons. Contact our technical support staff and we will be happy to design a strategy suitable for your genome editing project.


Details on Bioinformatics Analyses Services
Employing tools such as CRISPResso, abm is able to quickly identify all types of variations in the target sequence. With NGS data, abm will provide detailed sequence information and accurate edit ratio.

abm RNA Sequencing

Sample data from edited Genome

abm RNA Sequencing

Sample data from un-edited genome


Project Design/Advice Assistance
All of abm's sequencing services are performed by a group of specially trained and experienced scientists partaking in a streamlined workflow. Please contact our technical support team with details of your project requirements at [email protected] and our technical support team will be happy to work with you on confirming the details of your NGS order.


Confidentiality and Service Policy
All customer information is held in strict confidence. All materials and information sent to us and the products produced by us for the order are the property of the customer and will be returned to the customer or discarded in a confidential manner. We only archive customer materials when instructed to.

Our goal is always to deliver high-quality useful data to aid you in your research. We perform extensive maintenance and quality assurance on our equipment and strict quality control of samples throughout the sequencing process. However, next-generation sequencing by nature does not always deliver equally robust results due to a variety of factors, such as the variability inherent in the library preparation process, the biochemistry that takes place during sequencing and the quality of samples that we receive. We will make every effort to deliver the number of reads that are listed, although it is not possible to guarantee that those targets will always be achieved. The actual number of reads passing filter may vary by up to 10% from the listed target. Studies by various institutes have shown that there is minimal difference in coverage for 10% variation in reads past 5-10 million total reads.

All services are for research use only.

FAQs

Is your company certified as a service provider for Illumina Sequencing?
Yes, we are a certified Illumina service provider.
What kind of analysis software do you use for sequence analysis?
The Illumina sequencers come with the integrated software that can perform the regular bioinformatics analysis functions. In addition, we have extensive experience with other open access software such as Galaxy, Adnuril, BioBike, etc. In other words, we are willing to perform additional bioinformatics analysis with different software and tools to satisfy a variety of demands from our customers.
What kind of QC methods do you adopt for the customer's sample(s)?
We will perform QC on your Total RNA samples prior to sequencing them, using the Agilent Bioanalyzer (industry standard) to determine the RNA Integrity Number (RIN). If the RIN is lower than 8, we consider these samples as having failed QC. If the samples do not pass QC, we will ask you to send us new samples. If we do not receive new samples, thereby cancelling the sequencing order, there will be a QC fee of $50/sample. This fee will be waived if you choose to continue with the sequencing order. A strict positive and negative control will be applied to this QC process. The library QC will also be performed using the Agilent Bioanalyzer to determine library size and purity. Also, prior to loading the libraries on the sequencer, we perform qPCR quantification. The cost for this is included in the sequencing service. For the QC of the final data from our sequencing service, the integrated software will generally do the job and provide adequate QC information, however, we can also provide additional QC data such as FASTQC upon request. The data we output will pass our Q30 filter, which means that the error rate in base calls is less than 1 in 1000, or 0.1%. We will get a percentage at the end of the run which states the percentage of bases that have a Q score > 30, and this percentage is usually ≥85%.
What is the lead time for your WGS, WES, and RNA-Seq services?
Our sequencing lead times depend on workload, but are typically 2-4 weeks for sequencing data + 2-3 weeks for standard analysis. Please inquire for lead times for more detailed analysis.

Citations

01 Barclay, R. A. et al. "Exosomes from uninfected cells activate transcription of latent HIV-1." J. Biol. Chem 292(28):11682-11701 (2017). DOI: 10.1074/jbc.A117.793521. Application: RNA-Seq.
02 Ee, LS et al. "An Embryonic Stem Cell-Specific NuRD Complex Functions through Interaction with WDR5." Stem Cell Rep 8(6):1488-1496 (2017). DOI: 10.1016/j.stemcr.2017.04.020. Application: RNA-Seq.
03 Acharya, D et al. "KAT-Independent Gene Regulation by Tip60 Promotes ESC Self-Renewal but Not Pluripotency." Cell Rep 19(4):671-679 (2017). DOI: 10.1016/j.celrep.2017.04.001 .Application: RNA-Seq.
04 Goudarzi, R et al. "Effects of Arthrocen, an avocado/soy unsaponifiables agent, on inflammatory mediators and gene expression in human chondrocytes." FEBS Open Bio 7(2):187-194 (2017). DOI: 10.1002/2211-5463.12176. Application: RNA-Seq.
05 Taylor, J.F. et al. "In Vitro Effects of Arthrocen, an Avocado/Soy Unsaponifiables Agent, on Inflammation and Global Gene expression in Human Monocytes." IJC 9(4):31-39 (2017). DOI: 10.5539/ijc.v9n4p31. Application: RNA-Seq.
06 Alam, S.B. and Rochon, D. "Cucumber Necrosis Virus Recruits Cellular Heat Shock Protein 70 Homologs at Several Stages of Infection." J Virol 90 (7):3302-3317 (2016). DOI: 10.1128/JVI.02833-15. PubMed: 26719261. Application: RNA-Seq.
07 Ghoshala, K et al. "Encapsidation of Host RNAs by Cucumber Necrosis Virus Coat Protein during both Agroinfiltration and Infection." J Virol 89(21):10748-61 (2015). DOI: 10.1128/JVI.01466-15. PubMed: 26269190. Application: Next Generation Sequencing.
08 Nakamura, S et al. "Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation." Sci Rep 5:13474: (2015). DOI: 10.1038/srep13474. PubMed: 26310847. Application: Next Generation Sequencing.