PCR
As an industry-leading and global biotech company, abm offers a wide range of PCR, RT-PCR and qPCR enzyme products suitable for a variety of applications.
- PCR: Compared to regular Taq, our MegaFi™ Fidelity DNA Polymerase has 13000X better proofreading ability and our BlasTaq™ DNA polymerase is 3X faster.
- RT: Our OneScript® Reverse Transcriptases set a new benchmark for cDNA synthesis, with OneScript® Hot being the only reverse transcriptase in the world that preserves performance at 50-80˚ C.
- qPCR: Our polymerases are available as dye-based and probe-based MasterMixes, specially formulated for a variety of applications and premixed to streamline your workflows.
- Nucleic Acid Staining: Developed to replace toxic Ethidium Bromide, SafeView™ products are a new class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in both agarose and polyacrylamide gels.
- Specialized Kits: Real-time qPCR detection kits of viral particles for recombinant lentivirus, retrovirus and AAV, as well as PCR-based mycoplasma detection Kit for cell culture applications.
| Considering switching to abm but unsure which PCR Enzyme to switch to? Try our PCR Competitor Cross Reference Tool - simply select the current PCR brand and enzyme or MasterMix you are using and our tool will recommend the equivalent abm product. |
"I ordered some RT MasterMix and qPCR MasterMix. Both of them are very good, especially the BrightGreen qPCR Mastermix, which gives a very good signal and smaller cycle number than Sybrgreen. The price is cheaper as well. We will order more PCR reagents from ABM in the future."
Xiaoyun Tang, University of Alberta, BrightGreen 2X qPCR MasterMix-ROX and 5X All-In-One RT MasterMix
"I am very impressed with abm's RT MasterMix. It was very easy to use and the reaction time was very short."
Boram Ham, McGill University, Reverse Transcriptase MasterMix
Xiaoyun Tang, University of Alberta, BrightGreen 2X qPCR MasterMix-ROX and 5X All-In-One RT MasterMix
"I am very impressed with abm's RT MasterMix. It was very easy to use and the reaction time was very short."
Boram Ham, McGill University, Reverse Transcriptase MasterMix
Products
Reverse Transcription
Unsure which polymerase is right for you?
Unsure which polymerase is right for your application? Contact us for assistance or use the simple PCR Enzyme Selection Guide below to compare the characteristics of each polymerase.
| CHARACTERISTIC | MegaFi™ Pro Fidelity | BlasTaq™ | BlasTaq™ HotStart | Taq |
|---|---|---|---|---|
| Proofreading | Yes | No | No | No |
| Fidelity (vs. Native Taq) | 2000X | 1X | 1X | 1X |
| Specificity | •••• | ••• | •••• | ••• |
| Extension Speed | 20-30 sec/kb | 15 sec/kb | 15 sec/kb | 60 sec/kb |
| Target Length | 15-20 kb | 10-15 kb | 10-15 kb | 6.5 kb |
| Suitable for TA cloning | No | Yes | Yes | Yes |
| Special Feature | Fidelity and Sensitivity | Fast and Robust | High Specificity | For routine PCR |
| Cat. No. | G886 (Polymerase), G887 (PCR MasterMix) |
G894 (Polymerase), G895 (PCR MasterMix) |
G595 (Polymerase) | G009 (Polymerase), G888 (PCR MasterMix) |
Resources
-
Introduction to PCR
Polymerase Chain Reaction Knowledge Base -
DNA Polymerase Variation
Polymerase Chain Reaction Knowledge Base -
Real-Time PCR/Quantitative PCR (qPCR)
Polymerase Chain Reaction Knowledge Base -
Reverse Transcription (RT-PCR)
Polymerase Chain Reaction Knowledge Base
-
Variations to System
Polymerase Chain Reaction Knowledge Base -
PCR Product Guide (2021)
Catalog -
PCR/qPCR Competitor Cross Reference Tool
Top Publications
01
Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome
Wood, L. W., et al
Scientific Reports (2016) 6(19857)
doi: 10.1038/srep19857
02
Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid binding protein
Ly, D., et al
Scientific Reports (2016) 6(35651)
doi: 10.1038/srep35751
03
MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration
Antony, A., et al
Nature Communications (2016) 9(7):10955-65
doi: 10.1038/ncomms10955
Testimonials
I am very satisfied this product. It is easy to use and accurate. That's why I keep using them to check lentivirus titer. Thank you abm for developing this kit which makes my research easier.
Xinping Huang
Emory University
qPCR Lentivirus Titer Kit
I am a fan of abm: great product quality, great price, great rewards and great experience.
Dr. Vincent Emond
Centre de recherche du CHU de Québec CHUL
Safe-Green, Opti-Protein XL ladders and Safe-Green 100bp Opti-DNA ladders
I have recently switched to using this product and am very happy with the results. Bestaq is reliable, fast, easy to use, and competitively priced. I will be using Bestaq for the reminder of my cloning throughout my studies.
Heather MacKay
The University of British Columbia
Bestaq DNA Polymerase
Promotions
- Sku: G898
Price: $180.00
Unit: 20 rxnmiRNA All-In-One cDNA Synthesis Kit is a complete system for efficient synthesis of first strand miRNA from total RNA templates. This comprehensive kit comes with just two components: 2X miRNA cDNA Synthesis SuperMix and Enzyme Mix, enabling...
- Sku: G592
Price: $135.00
Unit: 100 rxn(Equivalent to Cat. No. G451, G452, G453, G454, G485, G486, G490, G492, G590, and G916.) Enjoy accurate gDNA-free cDNA synthesis! All-In-One 5X RT MasterMix is a convenient and ready-to-use formulation for first-strand cDNA synthesis, including...
- Sku: G895
Price: $245.00
Unit: 800 rxn (10.0 ml)(Equivalent to Cat. No. G014, G280, G465, G901 with or without dye.) Blast through a routine 2 hour PCR run in only 20 minutes! BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s BlasTaq™ DNA Polymerase in a...
- Sku: G892
Price: $495.00
Unit: 2,500 rxn (25.0 ml)(Equivalent to Cat. No. MasterMix-LR-XL, -R-XL, and S-XL.) BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix...
- Sku: G891
Price: $125.00
Unit: 500 rxn (4 x 1.25 ml)(Equivalent to Cat. No. MasterMix-5L, -5R, -5S, -EL, -ER, -ES, KL, -KR, -KS, -LR, -mL, -mR, -mS, -R, and -S) BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA...
- Sku: G894
Price: $125.00
Unit: 400 rxn(Equivalent to Cat. No. G012, G040, G277, and G278.) Blast through a routine 2 hour PCR run in only 20 minutes! BlasTaq™ DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates and robust...
- Sku: G595
Price: $295.00
Unit: 400 rxn(Equivalent to Cat. No. G011, G039, G937.) Eliminate non-specific amplification and primer-dimer formation! BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust...
- Sku: G890
Price: $125.00
Unit: 500 rxn (4 x 1.25 ml)(Equivalent to Cat. No. MasterMix-5P, -5PL, -5PM, -5PS, -P, -PL, -PM, and -PS.) BlasTaq™ Probe 2X qPCR MasterMix is designed for TaqMan probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix (dNTPs, BlasTaq™ DNA...
- Sku: G596
Price: $115.00
Unit: 100 rxn(Equivalent to Cat. No. G493-P, G493-PL, G493-PS, G918-P, G918-PL, and G918-PS.) BlasTaq™ Probe One-Step RT-qPCR provides convenience using the RT-qPCR Enzyme Mix and BlasTaq™ Probe 2X qPCR MasterMix for both highly sensitive and specific...
- Sku: G597
Price: $195.00
Unit: 100 rxn(Equivalent to Cat. No. G174 and G174-dye.) Simple One Step Reaction, Superior Performance! MegaFi™ One-Step RT-PCR is an enzyme mix of OneScript® Hot Reverse Transcriptase and MegaFi™ Fidelity DNA Polymerase with RNaseOFF Ribonuclease...