|Phone:||604-247-2416 (Local), 1-866-757-2414 (Toll free)|
|Payment:||or Purchase Order Number|
|More info:||Additional Information Regarding Orders|
|Our Basic Package (C195) Includes||Deliverables||Lead Time|
|Add-On Services||Quantity||Cat. No.||Price|
|Additional Clones||1 Clone||C143||$250.00|
|Additional Vials of Delivered Clones||1 Vial||C144||$80.00|
|Cell Line Authentication Service||1 Service||C287||$150.00|
If your cells require medium other than DMEM and RPMI, we will also require 1L of the specified medium and any applicable growth factors or supplements to be provided by you for the project. Please submit all components of the complete media (e.g. if growth factors, cytokines etc. are applicable) individually to eliminate potential degradation of components in the media during transit. Kindly include instructions for making the complete media in your shipment. Additionally, please provide at least 5 x coated 6-well plates and 10 x T25 flasks if the cells require specially treated culture vessels. Once you have shipped your cells, please forward us the tracking number for custom clearance. Information on how to ship cell samples to abm can be found on our support page: https://www.abmgood.com/Technical-Support.html
Once we receive the cells, we will performed custom cell immortalization in 6-well plates with various cell immortalization reagents, such as but not limited to, SV40 T antigen, hTERT, Myc, E6/E7.
We will make sure the cells are immortalized and have the ability to grow forever. The final product to be shipped to you with be up to two different clones that have been successfully immortalized.
Where applicable, the cells will be selected by antibiotics then subsequently passaged 30+ beyond the normal stage of senescence to confirm immortalization. We closely monitor typical cell behavior including growth rate and morphology., however all downstream characterization must be determined by the end user. We do not conduct gene expression assays as part of this service. Please note there may be sometimes changes in any markers or morphology due to immortalization and we do not claim that all or any specific markers be retained after immortalization.
QC data provided in a final custom service report will include:
- Morphology Assessment
- qPCR Analysis on the transgene expression
- Test results for presence/absence of microbial contaminants