Cell Immortalization
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Custom Cell Immortalization Service
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Cat.No.: C195
Quantity: Basic Service Package
Price: Inquire
Email: order@abmGood.com
Phone: 604-247-2416 (Local), 1-866-757-2414 (Toll free)
Fax: 604-247-2414
Payment: or Purchase Order Number
More info: Additional Information Regarding Orders



Orders of this service are subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at technical@abmgood.com. The end user acknowledges that the Materials provided under abm’s Material Transfer Agreement (MTA) does not grant a license for commercial use or imply any ownership rights of any intellectual property rights relating to the Materials.
Service Details

Our Basic Package (C195) Includes Deliverables Lead Time
  1. Viral transduction on primary cells submitted
  2. Cell immortalization
  3. Drug selection on successfully transduced cells
  4. Clone screening and expansion confirmation of immortalization via qRT-PCR of Transgene
  5. Cryopreservation and delivery of cells
  1. Up to two clones, 2 vials/clone
  2. Any additional add-ons where applicable
  3. Service report includes:
    • Morphology Assessment
    • qPCR Analysis of transgene expression
    • Test results for presence or absence of microbial contaminants including bacterial, fungal, and mycoplasma testing
Please Inquire

Add-On Services Quantity Cat. No. Price
Additional Clones 1 Clone C143 $250.00
Additional Vials of Delivered Clones 1 Vial C144 $80.00
Human Cell Line Authentication Service 1 Sample C287 $150.00


Follow these steps to order an immortalization service (quick view)
  1. To obtain a quote, please fill out the service questionnaire and submit to quotes@abmgood.com
  2. Place an order and specify the quote number during order placement. Note that samples received without an order confirmation number indicated will be disposed immediately upon receipt to ensure that all customer information is held in strict confidence.
  3. Once you have received an order confirmation number, please set up shipment and submit:
    • Cells: At least 2x106 cells/cell line. If you require more than 1 immortalization reagent, please submit an additional 1x106 cells/reagent.
    • Media: All necessary media components, including any applicable FBS, growth factors and/or supplements required for culturing (abm can only provide DMEM and RPMI if needed). Please submit all components of the complete media individually to eliminate potential degradation of components in the media during transit. Kindly include instructions for making the complete media in your shipment. Volume requirement: 1 L of complete media.
    • Flasks: At least 5 x coated 6-well plates and 10 x T25 flasks if the cells require specially treated culture vessels.
    • Cell morphology: Please supply a picture of your primary cells growing in culture to depict 'original' cell morphology when setting up shipment by contacting order@abmgood.com with Order XXXXX in the subject line. This is to help us assess the cells we receive are in good condition before we start the project. Information on how to ship cell samples to abm can be found here.
  4. abm will confirm cell morphology, immortalize your cells, and perform QC.
  With over 10 years of experience immortalizing cell lines, scientists at abm have been successful in immortalizing essentially every human and mouse cell type using different immortalizing agents or genes. We are expanding our expertise with mammalian cells and now offer this service to immortalize your requested cell lines. If you have a primary cell culture that you would like to immortalize, our custom services are the quickest and most reliable tool for your project, saving you the precious time and money you would have otherwise lost attempting different genes yourself. Our custom service package is designed to allow you to immortalize your cells using immortalization reagents selected from our offerings, enabling you to easily obtain your desired cell line for your research needs.

  We have many different options available to immortalize your cell line. Choose from 1-2 of our immortalization reagents. We will work to immortalize your cells using the desired reagents.

  Not sure which reagent is suitable for your cell line? Let our abm experts select the right reagents for your project. We design the immortalization procedure based on extensive scientific research and decades of experience.
Product Documents
Product References
FAQs
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How do I send my cells and place an order? and What will I be receiving?
For frozen cells, you must ship in ample dry ice and each vial must contain 2 million cells. Another method is to send us live cells. If the cells are fast growing, we recommend sending three T25 flasks at 70% density. If the cells are slow growing, we recommend sending two T25 flasks at 90% density. The flask should be filled with complete medium without any air bubble and at room temperature. The flask can be filled with medium using a 50ml culture tube. Make sure that sterilization procedure is strictly observed. For North American customers, the cells should be sent on Monday. For non-North American customers, the cells should be sent on Friday or Monday to avoid transit delay over the weekend. Please note that the cells are immortalized in the condition that they were received and we cannot be liable for any changes during transit. We ask that customers supply a picture of your primary cells growing in culture to depict 'original' cell morphology when setting up shipment. This is to help us assess the cells we receive are in good condition before we start the immortalization project.

If your cells require medium other than DMEM and RPMI, we will also require 1L of the specified medium and any applicable growth factors or supplements to be provided by you for the project. Please submit all components of the complete media (e.g. if growth factors, cytokines etc. are applicable) individually to eliminate potential degradation of components in the media during transit. Kindly include instructions for making the complete media in your shipment. Additionally, please provide at least 5 x coated 6-well plates and 10 x T25 flasks if the cells require specially treated culture vessels. Once you have shipped your cells, please forward us the tracking number for custom clearance. Information on how to ship cell samples to abm can be found on our support page: https://www.abmgood.com/Technical-Support.html

Once we receive the cells, we will performed custom cell immortalization in 6-well plates with various cell immortalization reagents, such as but not limited to, SV40 T antigen, hTERT, Myc, E6/E7.

We will make sure the cells are immortalized and have the ability to grow forever. The final product to be shipped to you with be up to two different clones that have been successfully immortalized.
Can I purchase immortalized human neurons? I don't have primary neurons, but rather you can provide both the primary cell and the immortalization service?
Yes, we will be able to try to immortalize those cells for you. Please let us know your specific requirement and we will send you an official quotation.
How many cells do I need to send in order to immortalize one cell line?
For frozen cells, you must ship in ample dry ice and each vial must contain 2 million cells. Another method is to send us live cells. If the cells are fast growing, we recommend sending three T25 flasks at 70% density. If the cells are slow growing, we recommend sending two T25 flasks at 90% density.
What should I do if my cells do not grow using standard media?
If your cells do not grow using DMEM or RPMI, then we need at least 1L of your growth media plus additional amounts of associated growth factors.
What kinds of methods are you going to take to demonstrate the lentivirus-infected cells are immortalized and whether the cells characteristics are changed after immortalization?
Usually if the cells show positive transgene expression in qPCR and are passaging well in comparison to the primary cells, that is a confirmation of immortalization. In one of the rare events, if the trangene expression is lost over some late passages, we may need to re-immortalize.

Where applicable, the cells will be selected by antibiotics then subsequently passaged 30+ beyond the normal stage of senescence to confirm immortalization. We closely monitor typical cell behavior including growth rate and morphology; however, all downstream characterization must be determined by the end user. We do not conduct gene expression assays as part of this service. Please note there may be sometimes changes in any markers or morphology due to immortalization and we do not claim that all or any specific markers be retained after immortalization.
Does the service perform western blot analysis or qPCR analysis in order to confirm the cells were successfully immortalized?
qPCR analysis is performed on the transgene expression
Please let us know details of the deliverables from this service.
Assuming at least 2 million cells are provided, the final deliverables will be up to 2 clones from a single reagent.

QC data provided in a final custom service report will include:
- Morphology Assessment
- qPCR Analysis on the transgene expression
- Test results for presence/absence of microbial contaminants