Probe Design & Synthesis Service

abm offers a one-stop TaqMan probe design service, aiming to save time, effort, and money from preventing complications or even failures in qPCR experiments. A well designed probe is one of the key requirements for a successful qPCR. Using appropriate software and virtual simulation, scientists at abm are able to design TaqMan probes with minimal predicted complication of secondary structure at the binding sequence region on the template, thus ensuring an effective binding and ultimately an efficient hydrolysis of the probe. However, the effectiveness of the TaqMan probe still needs to be tested experimentally and so this probe design service provides 2 probe designs, in case the first probe does not perform properly in the actual assay. In very rare cases, a re-design of probes will be provided without additional charges when both initially designed probes did not work in the assay.

"I am a fan of abm: great product quality, great price, great rewards and great experience."

Dr. Vincent Emond, Centre de recherche du CHU de Québec CHUL

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Service Details

Service NameQuantityCat. No.Price
TaqMan Probe Design Service 1 Target Design (2 Probes) C131 $250.00
Probe Synthesis (FAM/BHQ1) 100 nmol C134 $282.20
Probe Synthesis (FAM/BHQ1) 250 nmol C135 $387.70
Probe Synthesis (HEX/BHQ1) 100 nmol C136 $348.50
Probe Synthesis (HEX/BHQ1) 250 nmol C137 $492.50
Probe Synthesis (ROX/BHQ2) 250 nmol C331 $590.00
Probe Synthesis (Cy5/BHQ2) 100 nmol C332 $448.00
Probe Synthesis (Cy5/BHQ2) 250 nmol C333 $520.00
Probe Synthesis (FAM/TAMRA) 100 nmol C334 $282.00
Probe Synthesis (FAM/TAMRA) 250 nmol C335 $387.50

Additional Info

abm offers traditional dual-labelled probes for use in real-time PCR to increase efficiency, sensitivity and specificity, as well as allowing simultaneous detection of multiple gene targets in a single reaction. We offer qPCR probes in a variety of reporter/quencher combinations. The following table outlines our synthesis scale and the minimum guarantee OD yield for each service.

Cat. No5’ Reporter DyeQuencherSynthesis ScaleMin Guarantee OD yield
C134 6’FAM BHQ1 100 nmol 1.5 OD
C135 6’FAM BHQ1 250 nmol 3.0 OD
C136 HEX BHQ1 100 nmol 1.5 OD
C137 HEX BHQ1 250 nmol 3.0 OD
C331 ROX BHQ2 250 nmol 3.0 OD
C332 Cy5 BHQ2 100 nmol 1.5 OD
C333 Cy5 BHQ2 250 nmol 3.0 OD
C334 6'FAM TAMRA 100 nmol 1.5 OD
C335 6'FAM TAMRA 250 nmol 3.0 OD
1. The synthesis scale indicates the initial amount of 3'-bases.
2. Average OD yield was determined for a 20-mer; Calculation: 1 OD = 5 nmol = 30 ug; may vary for sequences with GC content >70%, >3 purine stretches, or strong secondary structures.
All probes are HPLC purified and are delivered in lyophilized powder in tube format. abm understands the necessity of good probe designs and our design is thoroughly evaluated against bad probe designs:

Probe Design Synthesis

Order Instructions
To place an order, download and fill out an Probe Order Form. Please include all the required information such as your probe name, probe sequence, 5’- and 3’- modifications and the synthesis scale. Email the Order Request Form to [email protected]. A confirmation email will be sent to your email address shortly.


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