RNase H, E. coli

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RNase H, E. coli
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Cat.No.: E018
Quantity: 500 U (100 μl)
Price: $25.00
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Data Sheet
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Description RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase H does not digest single-stranded or double-stranded DNA and RNA.
Application
  • Removal of the poly(A) sequences from mRNA in the presence of oligo (dT)
  • Directed cleavage of RNA

  • Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
    Form Enzyme supplied with 10X Reaction Buffer
    Image
    Concentration 5 U/μl
    Storage Store all components at -20°C.
    Storage Buffer 20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol.
    Notes One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT).

    This product is distributed for laboratory research only.
    Caution: Not for diagnostic use.
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