RNase III, E.coli

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RNase III, E.coli
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Cat.No.: E020
Quantity: 100 U (100 μl)
Price: $85.00
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Data Sheet
Print Version
Description RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3´-overhangs with 5' phosphate and 3' hydroxyl termini.
Application • dsRNA digestion to short dsRNA fragments
• Useful for RNA processing, maturation and structure studies
• Directed cleavage of RNA
• siRNA preparation for RNA interference in mammalian cells

Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Form Enzyme supplied with 10X Reaction Buffer
Image
Concentration 1 U/μl
Storage Store at -20°C.
Storage Buffer 50 mM Tris-HCl (pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Notes One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.

This product is distributed for laboratory research only.
Caution: Not for diagnostic use.
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