Site Directed Mutagenesis Service
abm has over 10 years’ experience with Site-Directed Mutagenesis Services and we have successfully completed hundreds of orders. Past projects include modifying a DNA construct, such as alteration of a restriction site in a vector, insertion or deletion of vector elements, tag addition/removal, base substitution, sequence deletion/insertion and promoter insertion/swapping. For large plasmids, large fragment insertions/deletions, difficult templates, and other mutagenesis requests, please inquire.
|Delivery Format||Plasmid DNA|
|Lead Time||In as little as 2-3 weeks for orders of 1.0 μg of DNA|
|Quality Control||Full-length sequencing to confirm presence of mutation|
|DNA Submission Guidelines||At least 10μg DNA per mutated clone required (200ng/μl or higher). Plasmids must be high copy plasmids. For medium or low copy plasmids, please inquire.|
"Our lab has used abm's site-directed mutagenesis service many times, and you always produce great plasmids!"Stuart Jarrett, University of Kentucky
|Service Name||Unit||Cat. No.||PRICE|
|Site-Directed Mutagenesis Service||Single Clone||C047||$150.00|
|Service NName||Unit||Cat. No.||Unit NPrice|
|DNA Sequencing||Single Reaction||C199||$7.50|
|DNA Sequencing with Custom Primer Synthesis||Single Reaction||C197||$13.50|
|Bacterial Glycerol Stock||1 clone||C139||$25.00|
|Plasmid Amplification (10-20 μg)||10‑20 μg||C309||$75.00/prep|
|Plasmid Amplification (80-100 μg)||80‑100 μg||C310||$150.00/prep|
*Fragments over 2kb are subject to an additional charge of $100.00/kb (C100).
Additional InfoComing Soon
|Does this also apply for insertions/deletions/mutations that span >25 consecutive base pairs?|
Pricing for Cat# C047 only applies for insertions/deletions/mutations that span <25 consecutive base pairs or fewer. Additional charges may apply for any insertions/deletions/mutations above the initial 25bp.
|01||Wen, Z et al. "MicroRNA-377 Regulates Mesenchymal Stem Cell-Induced Angiogenesis in Ischemic Hearts by Targeting V." PLoS One 9(9):e104666 (2014). DOI: 10.1371/journal.pone.0104666 PubMed: 25251394 Application: Inhibitor miRNA lentivector.|
|02||Laryea, G et al. "Site-specific modulation of brain glucocorticoid receptor and corticotropin-releasing hormone expression using lentiviral vectors." Mol Cell Endocrinol 371(1-2):160-5 (2013). DOI: 23261985 PubMed: 10.1016/j.mce.2012.12.005 Application: EF1a lentivector.|
|03||Das, A et al. "Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation.." J Immunol 192(3):1120-9 (2014). DOI: 10.4049/jimmunol.1300613 PubMed: 24391209 Application: Transduction.|
|04||Eagleman, DE et al." Unbiased proteomic screening identifies a novel role for the E3 ubiquitin ligase Nedd4-2 in translational suppression during ER stress." J Neurochem 157(6):1809-1820 (2021). DOI: 10.1111/jnc.15219 PubMed: 33064840 Application: Site Directed Mutagenesis.|