Site Directed Mutagenesis Service

Site Directed Mutagenesis Service
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Cat.No.: C047
Quantity: Single Clone
Price: $150.00
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Phone: 604-247-2416 (Local), 1-866-757-2414 (Toll free)
Fax: 604-247-2414
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More info: Additional Information Regarding Orders
Cat. No. Product Name Qty. Promo/% Off Amount
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Haul in savings with our December-only offers on restriction enzymes, growth factors, PCR products and more! Check in every week of December to unwrap a new gift! Details here:
*Notes about Promo Codes: For NON-DISCOUNT promo codes (i.e. free items or shipping) please enter them during check out in the Notes/Promo Code field. To enter a DISCOUNT promo code enter it into the corresponding box in the 'Promo/% Off' column and then click 'Update' for the given product. If the code is valid for that product the discount percentage will display in place of the box and the amount will become the discounted total for that product(s). If the code is not valid it will remain in the box, please double check the expiry and spelling, and if you feel the code should still be accepted please contact us.
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Service Details
Site-directed mutagenesis is a process of creating specific alterations in a gene. This technique has been used to study the relationship of protein structure and its function. Using site-directed mutagenesis, a gene can be modified by base substitution, sequence deletion and insertion. Site-directed mutagenesis is also very useful for modifying a DNA construct, such as alteration of a restriction site in a vector, insertion or deletion of vector elements, tag addition, tag removal and promoter insertion and exchange. Please inquire for a quote for large plasmids, large fragment insertions/deletions, difficult templates, and multiple mutagenesis.
- perform PCR-based mutagenesis
- deliver plasmid DNA
- turn-around time: 10-15 business days for orders of 2ug of DNA
- full length sequencing to confirm presence of mutation
- mutate up to 25bp (consecutive) within a <2kb fragment in any existing abm product or a provided vector. *Fragments over 2kb are subject to an additional charge of $100.00/kb (C100).
DNA Submission Requirements
Please provide at least 10ug DNA per mutated clone required (200ng/ul or higher). Plasmids must be high copy plasmids. For medium or low copy plasmids, this may incur an additional fee, please inquire with
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Does this also apply for insertions/deletions/mutations that span >25 base pairs consecutive?
The pricing for Cat# C047 only applies for insertions/deletions/mutations that span ≤25 base pairs consecutive. Additional charges may apply for any insertions/deletions/mutations above the initial 25bp.