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Site-directed mutagenesis is a process of creating specific alterations in a gene. This technique has been used to study the relationship of protein structure and its function. Using site-directed mutagenesis, a gene can be modified by base substitution, sequence deletion and insertion. Site-directed mutagenesis is also very useful for modifying a DNA construct, such as alteration of a restriction site in a vector, insertion or deletion of vector elements, tag addition, tag removal and promoter insertion and exchange. Please inquire for a quote for large plasmids, large fragment insertions/deletions, difficult templates, and multiple mutagenesis.
- perform PCR-based mutagenesis
- deliver plasmid DNA
- turn-around time: 10-15 business days for orders of 2ug of DNA
- full length sequencing to confirm presence of mutation
- mutate up to 25bp (consecutive) within a <2kb fragment in any existing abm product or a provided vector. *Fragments over 2kb are subject to an additional charge of $100.00/kb (C100).
How much DNA do I need to provide for this service?
Please provide 5ug DNA per mutated clone required (200ng/ul or higher).
Does this also apply for insertions/deletions/mutations that span >25 base pairs consecutive?
The pricing for Cat# C047 only applies for insertions/deletions/mutations that span ≤25 base pairs consecutive. Additional charges may apply for any insertions/deletions/mutations above the initial 25bp.