Site Directed Mutagenesis Service

abm has over 10 years’ experience with Site-Directed Mutagenesis Services and we have successfully completed hundreds of orders. Past projects include modifying a DNA construct, such as alteration of a restriction site in a vector, insertion or deletion of vector elements, tag addition/removal, base substitution, sequence deletion/insertion and promoter insertion/swapping. For large plasmids, large fragment insertions/deletions, difficult templates, and other mutagenesis requests, please inquire.

Service
Procedure PCR-Based Mutagenesis
Delivery Format Plasmid DNA
Lead Time In as little as 2-3 weeks for orders of 1.0 μg of DNA
Quality Control Full-length sequencing to confirm presence of mutation
DNA Submission Guidelines At least 10μg DNA per mutated clone required (200ng/μl or higher). Plasmids must be high copy plasmids. For medium or low copy plasmids, please inquire.



"Our lab has used abm's site-directed mutagenesis service many times, and you always produce great plasmids!"

Stuart Jarrett, University of Kentucky

Additional Resources:

Service Details

Core Services
Service NameUnitCat. No.PRICE
Site-Directed Mutagenesis Service Single Clone C047 $150.00
Additional Services
Service NNameUnitCat. No.Unit NPrice
DNA Sequencing Single Reaction C199 $7.50
DNA Sequencing with Custom Primer Synthesis Single Reaction C197 $13.50
Bacterial Glycerol Stock 1 clone C139 $25.00
Plasmid Amplification (10-20 μg) 10‑20 μg C309 $75.00/prep
Plasmid Amplification (80-100 μg) 80‑100 μg C310 $150.00/prep
Mutate up to 25bp (consecutive) within a <2kb fragment in any existing abm product or a provided vector.
*Fragments over 2kb are subject to an additional charge of $100.00/kb (C100).

Additional Info

Coming Soon

FAQs

Does this also apply for insertions/deletions/mutations that span >25 consecutive base pairs?
Pricing for Cat# C047 only applies for insertions/deletions/mutations that span <25 consecutive base pairs or fewer. Additional charges may apply for any insertions/deletions/mutations above the initial 25bp.

Citations

01 Wen, Z et al. "MicroRNA-377 Regulates Mesenchymal Stem Cell-Induced Angiogenesis in Ischemic Hearts by Targeting V." PLoS One 9(9):e104666 (2014). DOI: 10.1371/journal.pone.0104666 PubMed: 25251394 Application: Inhibitor miRNA lentivector.
02 Laryea, G et al. "Site-specific modulation of brain glucocorticoid receptor and corticotropin-releasing hormone expression using lentiviral vectors." Mol Cell Endocrinol 371(1-2):160-5 (2013). DOI: 23261985 PubMed: 10.1016/j.mce.2012.12.005 Application: EF1a lentivector.
03 Das, A et al. "Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation.." J Immunol 192(3):1120-9 (2014). DOI: 10.4049/jimmunol.1300613 PubMed: 24391209 Application: Transduction.