SpeedySeq DNA Sequencing
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SpeedySeq DNA Sequencing
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Cat.No.: C199
Quantity: 1 Sample
Price: $5.50
Qty.:
Email: order@abmGood.com
Phone: 604-247-2416 (Local), 1-866-757-2414 (Toll free)
Fax: 604-247-2414
Payment: or Purchase Order Number
More info: Additional Information Regarding Orders

Note: If you require custom primer synthesis with your SpeedySeq DNA Sequencing, please order SpeedySeq DNA Sequencing with Custom Primer Synthesis, C197 instead.
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Service Details
Description
  ABM is proud to launch a new and express DNA sequencing service for our valued customers. This service has been designed for rapid delivery and great value, making your sequence data available for download within a week of your samples being received.
  We employ streamlined technology platforms along with our proprietary reagents for an optimized and highly accurate performance, with on-hand tech support to assist with your primer design and special requirements - you can count on our dedicated and highly experienced team for a reliable service to meet all your DNA sequencing needs.
  In addition to our sequencing service, we also provide custom oligonucleotide synthesis - making your sequencing project easier than ever before, please refer to our SpeedySeq DNA Sequencing with Custom Primer Synthesis, C197 for included primer synthesis.
Features/Specifications
DNA Sequencing of templates up to 800bp - results delivered within a week.

Submit a wide range of DNA samples:
  • purified PCR products
  • plasmids
  • genomic DNA
  • single-stranded Phage DNA
  • large constructs (DNA, BAC, Cosmids and YAC clones)

  • Select your free primers from our list of available universal primers, use our oligonucleotide synthesis service for unique primer design or send your own primers along with your samples.

    We can solve many sequencing related problems, such as multiple hairpin loops and uneven base distributions.

    We perform rigorous quality control checks to maintain a reliable service.
    How to Use the Service
    1.   Download and fill out an order request form. Please include all the required information such as your template name, concentration, estimated size and primer name, plus any special notes (high/low GC, multiple hairpin etc). Please provide your billing information & Email address. You will be sent a unique password to your email to access your sequencing data online.
        Order request form can be attached and emailed to order@abmgood. A confirmation email for shipping your sample will then be sent to your email address. If a primer name is listed on the ABM Sequencing Primer list, these will be provided as part of the service at no additional cost. When choosing our free sequencing primer(s), please use the primer name exactly as shown in the list, and leave the primer concentration cell blank. If they are not listed, they will need to be provided along with your samples.

    2. Send your samples (+ Primers where applicable) to us at:
      ABM Inc.
      1-3671 Viking Way
      Richmond, BC
      V6V 2J5 CANADA

      Template Type DNA Concentration DNA Volume Primer Concentration Primer Volume
      Plasmid 100 ng/μl 10 μl 5 μM 10 μl
      PCR (100-500bp) 5-10 ng/μl 10 μl 5 μM 10 μl
      PCR (500bp-1Kb) 10-20 ng/μl 10 μl 5 μM 10 μl
      PCR (>1.0Kb) 20-40 ng/μl 10 μl 5 μM 10 μl
      Cosmid 0.5-1 μg/μl 20 μl 5 μM 10 μl
      Fosmid 0.5-1 μg/μl 20 μl 5 μM 10 μl
      BAC 0.5-1 μg/μl 20 μl 5 μM 10 μl
      Genomic DNA 2-3 μg/μl 20 μl 5 μM 10 μl

    3. You will receive an email containing your login name and password along with instructions on how to download your sequencing data via the DNA Sequencing Results Login
    abm's DNA Sequencing Primers (Free of Charge)
    We are continually adding new sequencing primers to our repertoire, please view the available sequencing primers list below to find out which primers we currently have available in house.
    Product Documents
    Product References
    FAQs
    Submit a new question:
      Question:
      Email:        
    Do you pay the transport of the samples?
    We do not cover the cost of shipping samples to our sequencing facility, and this is to be arranged by the end user. If you have any queries regarding shipment of DNA samples, please email technical@abmgood.com for a prompt response.

    Please note that pricing is per sample with 1 primer. For the same sample with 2 primers, the cost will be 2 x C199.
    I plan to send plasmids for sequencing, what is the lowest conc. for submission? Some plasmids were just around 20ng/ul. Do I need to condense my plasmids?
    The minimum requirement for submission of plasmid DNA for sequencing is 100ng/ul. If you require any further assistance or information please contact technical@abmgood.com
    What is the link for the software to read the results and how do I determine the “N” reads with the most probable sequence for that nucleotide?
    The software (FinchTV) for reading the chromatograms can be downloaded for free from Geospiza:
    http://www.geospiza.com/Products/finchtv.shtml

    When the software is downloaded and installed, all you have to do is open the chromatogram.
    ABI format chromatogram file (.ab1)

    The bases and position numbers will be shown at the top of the chromatogram, so you can easily find where the N is located and check the peaks to see what the nucleotide should be.
    How do I open the files?
    You may open .seq files, which contains the raw sequence data, using Notepad. For chromatograms, you will need to install FinchTV (http://www.geospiza.com/ftvdlinfo.html) to open .ab1 files, which contains the visualization of the sequencing reactions.
    Am I able to submit a sample that is 1500bp?
    Yes, you can. For 1500bp sequences, you will need to provide 2 primers to cover the whole sequence, instead of 1. The service cost for this sample would be a quantity of 2 x C199.