One of the key features of recombinant technology is that it provides an elegant way to fuse a recombinant protein with a protein/peptide tag, a choice that pivots on the role that is expected of the tag. For example, an epitope tag can aid the solubilization and cellular localization for the fusion recombinant protein and/or greatly simplify the process of purification through the use of tag-specific antibodies. Commonly used epitope tags include His-tag (6-Histidine), HA tag (hemagglutinin), cMYC tag, GST (glutathione-S-transferase) tag, and FLAG tag (DYKDDDDK). abm offers a wide array of anti-tag antibodies, each of which exhibit high specificity towards their associated epitope tags irrespective of the sequence of adjacent/nearby amino acids. Note that both monoclonal and polyclonal tag-specific antibodies are available for use in various applications, such as Western Blotting, ELISA, immunoprecipitation and immunofluorescence.
For lysates please see our Tag Lysates page.