AAV Blank Control Vector (EF1a) (Amp)
AAV are stable at room temperature for short term storage and are shipped with gel ice packs. Do not store at -20°C or 4°C for long term. For long term storage, it is recommended to store the viruses at -80°C and avoid repeated freeze-thaw cycles. Before storing at -80°C, it is advisable to make small aliquots to avoid repeated freeze-thaw cycles.
1) Disclaimer for Transcript Variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final.
abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data or a western blot to evaluate the level of gene expression. The replacement will not be covered by the same guarantee. Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user's experimental conditions.
|Are your pPB protein expression vectors high or low copy number plasmids?|
Our protein expression vectors are medium copy number plasmids and can be amplified using any standard miniprep or midi/maxi prep kits. There is no standard protocol that fits all proteins, therefore recombinant protein expression will need to be optimized and determined experimentally.
|When the protein is expressed from this vector, which enzyme do I need to use to remove my tag?|
The enzyme required to remove the tag (if possible) will depend upon the vector backbone corresponding to your product: 1) pPB-C-His: the His tag is NOT cleavable. 2) pPB-His-GST: the His-GST tag can be cleaved using TEV protease. 3) pPB-His-MBP: the His-MBP tag can be cleaved using TEV protease. 4) pPB-N-His: the His tag can be cleaved with Thrombin. 5) pPM-C-HA: the HA tag is NOT cleavable. 6) pPM-C-His: the His tag is NOT cleavable. 7) pPM-N-D-C-HA: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal HA tag is NOT cleavable. 8) pPM-N-D-C-His: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal His tag is NOT cleavable. Please see the following page for further details: https://www.abmgood.com/Protein-Vector.html
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