Blank-Control Protein Vector
CAT.NO | UNIT | PRICE |
---|---|---|
PV001 | 500 ng | $85.00 |
SKU | PV001 |
---|---|
Unit quantity | 500 ng |
Accession Number | N/A |
Format | Vector |
System | Protein Vector |
Vector Map |
Are your pPB protein expression vectors high or low copy number plasmids? | |
Our protein expression vectors are medium copy number plasmids and can be amplified using any standard miniprep or midi/maxi prep kits.
There is no standard protocol that fits all proteins, therefore recombinant protein expression will need to be optimized and determined experimentally. |
When the protein is expressed from this vector, which enzyme do I need to use to remove my tag? | |
The enzyme required to remove the tag (if possible) will depend upon the vector backbone corresponding to your product:
1) pPB-C-His: the His tag is NOT cleavable.
2) pPB-His-GST: the His-GST tag can be cleaved using TEV protease.
3) pPB-His-MBP: the His-MBP tag can be cleaved using TEV protease.
4) pPB-N-His: the His tag can be cleaved with Thrombin.
5) pPM-C-HA: the HA tag is NOT cleavable.
6) pPM-C-His: the His tag is NOT cleavable.
7) pPM-N-D-C-HA: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal HA tag is NOT cleavable.
8) pPM-N-D-C-His: the N-terminal D-tag can be cleaved using TEV protease. The C-terminal His tag is NOT cleavable.
Please see the following page for further details:
https://www.abmgood.com/Protein-Vector.html |
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