Column-Pure Plasmid Mini-Prep Kit

Cat. No.
D514
Unit
100 Preparations
Price
$110.00
Cat. No. D514
Name Column-Pure Plasmid Mini-Prep Kit
Unit 100 Preparations
Description

This miniprep column kit utilizes the silica spin filter to purify plasmid DNA. It is the easiest method for isolation of plasmid DNA and produces high-yield plasmid DNA of automated sequence quality. It is the most widely used method in modern molecular biology laboratories. All necessary tubes are supplied. The recovered plasmid DNA has a 1.8-2.0 OD260/280 ratio and is ready for automated sequencing, restriction digestion, or other downstream applications. The purified plasmid DNA is primarily in the supercoiled form.

Equipment Required:

  • Microcentrifuge
  • Pipettes

Key Features:

  • 15 minute, easy-to-follow method
  • Optimized for use with small & large plasmids
  • All necessary tubes supplied
  • Value-priced
  • High Capacity, with up to 10μg of DNA per column
  • High-Throughput Compatible
Application

Purification and isolation of plasmid DNA, up to 40kb from bacterial cultures and cells to be used in any downstream applications, such as sequencing, PCR, transformation or restriction digestions.

Storage Condition

Store most components of the kit at room temperature, with the exception of RNase A. Kits can be stored for up to 24 months without showing any reduction in performance and quality. RNase A stock solution can be stored for 2 years at 4°C. After addition of RNase A, Solution I is stable for 6 months at 4°C.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. D514
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  • Yang, Z., Tang, X., Hasing, M. E., Pang, X., Ghosh, S., McMullen, T. P. W., Brindley, D. N., & Hemmings, D. G. (2022). Human Cytomegalovirus Seropositivity and Viral DNA in Breast Tumors Are Associated with Poor Patient Prognosis. Cancers, 14(5), 1148. https://doi.org/10.3390/cancers14051148
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