GenomeCoV19 Detection Kit

G628100 Rxns

Customize GenomeCoV19 Detection Kit
1 x COVID-19 Primers/Probes   + $0.00
1 x 2X RT-qPCR MasterMix   + $0.00
1 x Positive Control Template   + $0.00
1 x Negative Extraction Control   + $0.00
1 x qRT-PCR Enzyme Mix   + $0.00
1 x Nuclease-Free H2O   + $0.00

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GenomeCoV19 Detection Kit
GenomeCoV19 Detection Kit

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    The most affordable qPCR Detection Kit for SARS-CoV-2 on the market.

    abm’s GenomeCoV19 Detection Kit is a real-time reverse transcription-polymerase chain reaction (RT-qPCR) test intended for the qualitative detection of RNA from SARS-CoV-2 in human nasopharyngeal and oropharyngeal swab specimens from individuals suspected of COVID-19 by their healthcare provider. Our GenomeCoV19 Detection Kit is the most affordable qPCR detection kit on the market at only $1.77 USD/test. 

    This kit is widely used in Europe under the CE-IVD certification. 

    GenomeCoV19 Detection Kit Features


    An ideal tool to detect COVID-19/ SARS-CoV-2 by RT-qPCR method

    Product Features• Highly specific for the RdRP and N target markers recommended by WHO and US CDC
    • Ready-to-use reaction kit that includes MasterMix, Primers/Probes, and Internal Positive Control
    • Can be used with samples collected from naso/oropharyngeal swabs
    • Results ready in less than 2 hours
    • Compatible with standard RT-qPCR machines (Bio-Rad CFX96, Flex 7TM QuantStudio, Agilent's Mx3005P etc.)
    Protocol OverviewTo help researchers in the global fight against the coronavirus, abm has developed an RT-qPCR Rapid Detection Kit for COVID-19 / SARS-CoV-2 that can be used on human respiratory tract specimens.

    Target genes:
    • RdRP and N genes as recommended by WHO and the US CDC

    • Results in less than 2 hours
    • Includes MasterMix, Primers/Probes, Internal Positive Control and Negative Extraction Control
    • 100 Reactions/Kit

    Kit components:
    • Mastermix
    • Primers/Probes
    • Positive Control
    • Negative Extraction Control

    Specimen types:
    • Naso/Oropharyngeal swabs

    This product is CE-IVD certified. For professional use only. This product is not licensed for sale in Canada and the United States of America.

    Storage Condition

    -25 to -15°C

    Unit quantity100 Rxns

    Variants detected: Through in silico analysis, our GenomeCoV19 Detection Kits (Cat. No. G628 and G628.v2) are able to detect the following new variants of the SARS-CoV-2 virus:

    SARS-CoV-2 Variant DetectedAs Defined By
    Alpha / UK variant B.1.1.7 Defined by the following mutations of the virus's spike proteins: deletion of 69-70, deletion of 144, N501Y, A570D, D614G, P681H, T716I, S982A, and, D1118H.
    Beta / South African variant B.1.351 Defined by the following mutations of the virus’s spike proteins: L18F, D80A, D215G, R246I, K417N, E484K, N501Y, and A701V.
    Brazilian variant B.1.1.28 Defined by the following mutations of the virus's spike proteins: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, H655Y, and, T1027I.
    Gamma / Brazilian variant P.1 (a branch off of the B.1.1.28 lineage) Defined by the following mutations of the virus's spike proteins: K417T, E484K, and, N501Y.
    Delta / Indian variant B.1.617.2 Defined by the following mutations of the virus’s spike proteins: L452R, T478K, D614G, P681R, D950N, and deletion of 156 -157.
    Delta Plus (a branch off of the B.1.617.2 lineage) Defined by the following mutations of the virus’s spike proteins: L452R, T478K, D614G, P681R, D950N, deletion of 156 -157, and K417N.
    Omicron / Variant B.1.1.529 Defined by the following mutations of the virus’s spike proteins: A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493K, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, and L981F.


    Limit of Detection (LoD):

    Our LoD study established the lowest concentration of SARS-CoV-2 RNA, as genome copies (cp) per reaction, which can be detected by the GenomeCoV19 Detection Kit at least 95% of the time. Positive reference sample was tested using both positive control template (IDT, Cat 10006625) and in vitro transcribed RNA control (IVT-RNA) from in house. The initial LoD was determined by testing template at 5000, 500, 50 and 5 cp/reaction in quadruplicate. The final LoD was confirmed by testing additional 20 replicates of samples at 5 cp/reaction (20/20 positive results). At the LoD threshold, the Ct was observed to have a mean of 32.72 (SD 0.43, % CV 1.32). The LoD was determined to be 5 cp/reaction.

    Clinical Performance (Contrived Samples):

    A contrived clinical study was conducted to evaluate the clinical performance. A total of 60 individual nasopharyngeal clinical specimens, collected from volunteers and presumed negative for SARS-CoV-2, were used in this study. A total of 30 negative and 30 contrived positive samples were tested. Positive samples were prepared by spiking BEI ATCC Genomic RNA from SARS Related Coronavirus 2 (Catalog No. NR-52285) into NP matrix mixed with lysis buffer from QIAamp DSP Viral RNA Mini Kit at 1X, 10X, and 100X LoD. Results showed 100% Positive Percent Agreement (30/30 positive results) and 100% Negative Percent Agreement (30/30 negative results).

    Inclusivity (Analytical Sensitivity):

    The primers and probes sequences were blasted against SARS-CoV-2 genomes publicly available as of on March 20th, 2020. Results showed the sequences had 100% homology to all SARS-CoV-2 isolates analyzed, with two exceptions of MT159720.1 and MT258382.1 (homology of 94% and 95% respectively). Both exceptions occur at the 5'end of the primer, and thus are unlikely to cause the failure of qPCR, and would not affect the test performance under specified annealing temperature.


    The detection results of this kit displayed no cross-reactivity with influenza A virus, influenza B virus, adenovirus, Staphylococcus epidermis, HCoV-SARS, HCoV-OC43, HCoV-HKU1, or Streptococcus pneumonia.

    Precision: CV < 5% (Between and within batches).

    DisclaimerFor in vitro diagnostic use only. For professional use only. This product is not licensed for sale in Canada.

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