DNAfectin™ 2100 Transfection Reagent

CAT.NOUNITPRICE
G21001.0 mg/1.0 ml
$145.00

Specifications


Description

DNAfectin™ Transfection Reagent is a special and unique formulation of polycations, offering a high transfection efficiency with low cytotoxicity for many cell types, including primary cells.

SKUG2100
Applications

Transfection of DNA into cultured eukaryotic cells.

Transfection Reagents TypeDNA Molecule
Unit quantity1.0 mg/1.0 ml
Note

Transfection Database

Our Transfection Cell Database contains data from transduction results from cell lines tested by in house scientists at abm. This is a continuing effort and more cell lines will be added to the database once the data is available. Please search our database below for specific details. If you have transfection results for cell lines that are not listed below, email us at [email protected] or fill in our online feedback form to receive +400 BONUS rewards points which you can exchange for gift cards and more!

 

Cell LineSpecies/TissueNucleic AcidReagentTransfection TypeTransfection Efficiency(%)
293T Human Embryonic Kidney DNA DNAfectin™ 2100 Transfection Reagent Transient 100%
Jurkat Human T Lymphoma DNA DNAfectin™ 2100 Transfection Reagent Transient 25%
SHEP Human Neuroblastoma DNA DNAfectin™ 2100 Transfection Reagent Transient 90%
BOSC23 Human Kidney DNA DNAfectin™ 2100 Transfection Reagent Transient 90%
HT1080 Human Fibrosarcoma DNA DNAfectin™ 2100 Transfection Reagent Transient 80%
C33A Human Cervical Carcinoma DNA DNAfectin™ 2100 Transfection Reagent Transient 90%
MRC5 Human Primary Fibroblast DNA DNAfectin™ 2100 Transfection Reagent Transient 80%
MDK-Telo Dog Kidney Cells+Telo DNA DNAfectin™ 2100 Transfection Reagent Transient 80%
B16 Murine Melanoma DNA DNAfectin™ 2100 Transfection Reagent Transient 80%
CB3 Mouse Erytholeukemia DNA DNAfectin™ 2100 Transfection Reagent Transient 25%
HuVEC Human Umbilical Vein DNA DNAfectin™ 2100 Transfection Reagent Transient 20%
HepG2 Human Liver Carcinoma DNA DNAfectin™ 2100 Transfection Reagent Transient 40%
MMRU Human Melanoma DNA DNAfectin™ 2100 Transfection Reagent Transient 75%
MMAN Human Melanoma DNA DNAfectin™ 2100 Transfection Reagent Transient 80%
MDA-MB-231 Human Breast Carcinoma DNA DNAfectin™ 2100 Transfection Reagent Transient 85%
HeLa Human Cervical Carcinoma DNA DNAfectin™ 2100 Transfection Reagent Transient 80%-90%
A2780 Human Ovarian Carcinoma DNA DNAfectin™ 2100 Transfection Reagent Transient 70%-75%
L6 Rat Myoblast DNA DNAfectin™ 2100 Transfection Reagent Transient 60%-70%
Rabbit-telo Telomerase-Rabbit Cells DNA DNAfectin™ 2100 Transfection Reagent Transient 50%
USO2 (T-USO2) Human Osteosarcoma (tet-inducible) DNA DNAfectin™ 2100 Transfection Reagent Transient 80%-90%
Storage Condition

Store at 4ºC. Do not freeze.

Documents


Supporting Protocol
MSDS

    QC

    Other
    FAQs


    How much transfection reagent and DNA do I need per well in a six well plate?
    5ug DNA and 10ul of transfection reagent will be fine. If toxicity is a problem, use 3~4 ug DNA.
    How much transfection reagent and DNA do I need for a 10 cm plate
    you will need 60~80ul depending how easy your cells can be transduced.
    Does freeze-thaw affect the performance of the transfection reagent?
    Freeze-thaw does damage the transfection reagent slightly, at an approximate 5% loss. If possible, test the agent with a GFP control first.
    References


    28
    • Tang, Y et al. "G-protein-coupled receptor for short-chain fatty acids suppresses colon cancer" International Journal of Cancer 128:847-856 (2010). DOI: 10.1002/ijc.25638. Application: Transfection.
    • Zhao, H et al. "Mitf is a transcriptional activator of medaka germ genes in culture" Biochimie 94:759-767 (2012). DOI: 10.1016/j.biochi.2011.11.007. Application: Transfection.
    • Sachdeva, M et al. "p53 represses c-Myc through induction of the tumor suppressor miR-145" PNAS 106:3207-3212 (2009). DOI: 10.1073/pnas.0808042106. PubMed: 19202062. Application: Transfection.
    • Jiang, Q et al. "Systematic validation of predicted microRNAs for cyclin D1" BMC Cancer 9:194 (2009). DOI: 10.1186/1471-2407-9-194. Application: Transfection.
    • Lavoie, G et al. "Phosphorylation of human DNMT1: Implication of cyclin-dependent kinases" Biochem. Biophys. Res. Commun. 409:187-192 (2011). DOI: 10.1016/j.bbrc.2011.04.115. Application: Transfection.
    • Wu, F et al. "MicroDNA-mediated Regulation of Ubc9 Expression in Cancer Cells" Clin Cancer Res 15:1550-1557 (2009). DOI: 10.1158/1078-0432.CCR-08-0820. PubMed: 19223510. Application: Transfection.
    • Sachdeva, M et al. "Negative regulation of miR-145 by C/EBP-b through the Akt pathway in cancer cells" Nucleic Acids Res. 40:6683-6692 (2012). DOI: 10.1093/nar/gks324. Application: Transfection.
    • Couillard, J et al. "5-Aza-20-deoxycytidine and interleukin-1 cooperate to regulate matrix metalloproteinase-3 gene expression" Int. J. Cancer 129:2083-2092 (2011). DOI: 10.1002/ijc.25865 . Application: Transfection.
    • Zhang, A et al. "The human long non-coding RNA-RoR is a p53 repressor in response to DNA damage" Cell Res. 23:340-50 (2013). DOI: 10.1038/cr.2012.164. PubMed: 23208419. Application: Transfection.
    • Sheth, S et al. "Resveratrol Reduces Prostate Cancer Growth and Metastasis by Inhibiting the Akt/ MicroRNA-21 Pathway" PLoS ONE 7:e51655 (2012). DOI: 10.1371/journal.pone.0051655. Application: Transfection.
    • Li, M et al. "Alternative transcription generates multiple Mitf isoforms with different expression patterns and activities in medaka" Pigment Cell Melanoma Res. : (2013). DOI: 10.1111/pcmr.12183. Application: Transfection.
    • Li, M et al. "Differential Evolution of Duplicated Medekafish mitf Genes" International Journal of Biological Sciences 9:496-508 (2013). DOI: 10.7150/ijbs.4668 . Application: Transfection.
    • Kaur, T et al. "Short interfering RNA against STAT1 attenuates cispatin-induced ototoxicity in the rat by suppressing inflammation" Cell Death and Disease 2:e180 (2011). DOI: 10.1038/cddis.2011.63. Application: Transfection.
    • Yuan, Y et al. "Correlative light and electron microscopic analyses of mitochondrial distribution in blastomeres of early fish embryos" FASEB J. : (2013). PubMed: 24136588. Application: Transfection.
    • Zhang , Z et al. "Negative regulation of IncRNA GAS5 by miR-21" Cell Death Differ. 20:1558-1568 (2013). DOI: 10.1038/cdd.2013.110. PubMed: 23933812. Application: Transfection.
    • Tang, Y et al. "Short-chain fatty acids induced autophagy serves as an adaptive strategy for retarding mitochondria-mediated apoptotic cell death" Cell Death and Differ. 18:602-618 (2011). DOI: 10.1038/cdd.2010.117 . PubMed: 20930850. Application: Transfection.
    • Peng, DF et al. "Loss of glutathione peroxidase 7 promotes TNF-α-induced NF-κB activation in Barrett's carcinogenesis" Carcinogenesis : (2014). PubMed: 24692067.
    • Zhu, S et al. "MicroRNA-21 targets tumor suppressor genes in invasion and metastasis" Cell Res 18:350-9 (2008). PubMed: 18270520.
    • Singh, R et al. "Exosome-mediated transfer of miR-10b promotes cell invasion in breast cancer" Molecular Cancer 13(1):256 (2014). DOI: 10.1186/1476-4598-13-256. PubMed: 25428807. Application: Transfection.
    • Soutto, M. et al. "Activation of β-catenin signalling by TFF1 loss promotes cell proliferation and gastric tumorigenesis" Gut : (0). PubMed: 25107557.
    • Zhu, et al. "Regulation of CD44E by DARPP-32-dependent activation of SRp20 splicing factor in gastric tumorigenesis" Oncogene :1-10 (2015). DOI: 10.1038/onc.2015.250.
    • Song, J et al. "miR-370 and miR-373 regulate the pathogenesis of osteoarthritis by modulating one-carbon metabolism via SHMT-2 and MECP-2, respectively" Aging Cell 5:826-837 (2015). DOI: DOI: 10.1111/acel.12363. PubMed: 26103880.
    • Zhu, S et al. "Regulation of CD44E by DARPP-32-dependent activation of SRp20 splicing factor in gastric tumorigenesis" Oncogene 14:1847-1856 (2016). PubMed: 26119931. Application: Transfection.
    • Zhu, S et al. "Regulation of CD44E by DARPP-32-dependent activation of SRp20 splicing factor in gastric tumorigenesis" Oncogene 14:1847-1856 (2016). PubMed: 26119931. Application: Transfection.
    • Ge, H., Zhao, Y., Shi, X., Tan, Z., Chi, X., He, M., … Li, H. "Squalene epoxidase promotes the proliferation and metastasis of lung squamous cell carcinoma cells though extracellular signal‐regulated kinase signaling" Thoracic Cancer 10(3):428–436 (2019). DOI: 10.1111/1759-7714.12944.
    • Jin, S. W., Alsahafi, N., Kuang, X. T., Swann, S. A., Toyoda, M., Göttlinger, H., ... & Brockman, M. A. "Natural HIV-1 Nef Polymorphisms Impair SERINC5 Downregulation Activity" Cell reports 29(6):1449-1457 (2019).
    • Ma, B., Zhang, J., Zhu, Z., Zhao, A., Zhou, Y., Ying, H., & Zhang, Q. "Luteolin Ameliorates Testis Injury and Blood–Testis Barrier Disruption through the Nrf2 Signaling Pathway and by Upregulating Cx43" Molecular nutrition & food research 1800843: (2019).
    • Peng, W. X., Koirala, P., Zhang, W., Ni, C., Wang, Z., Yang, L., & Mo, Y. Y. "LncRNA RMST enhances DNMT3 expression through interaction with HuR" Molecular Therapy. : (2019).