GOT2 Knockout Stable HEK293 Cell Line (Clone A7)

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T64261x106 cells / 1.0 ml
$0.00

Specifications


DescriptionCRISPR/Cas9 was used to introduce a disruption of the GOT2 gene in HEK293 cells. The resulting CRISPR/Cas9 GOT2-knockout HEK293 cell line is homozygous for 205dup.
SKUT6426
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemKidney
Cell MorphologyEpithelial
ApplicationsFor Research Use Only
Seeding Density30,000 - 50,000 cells/cm2
Population Doubling Time30 - 40 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfilelacZ-Zeocin; FRT integration site; GOT2 was knocked-out using pSpCas9(BB)-2A-GFP (PX458) (Addgene plasmid # 48138)
Preservation Protocol1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) Flow cytometry; 2) Western blot; 3) PCR; 4) Sanger sequencing
Documents


Supporting Protocol
MSDS

    QC

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