HeLa Stably Expressing HXBc2 tat (tat-III) Cell Line

CAT.NOUNITPRICE
T60201x106 cells / 1.0 ml
$0.00

Specifications


DescriptionHeLa Stably Expressing HXBc2 tat (tat-III) Cell Line is an effective tool for the trans-activation of transfected HIV-1 LTR. An in vitro cell line which can continuously propagate for a reliable source of source material for experiments; it is a valuable tool for research in HIV and the role of HCBc2 (gp120).
SKUT6020
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemReproductive
Growth PropertiesLoosely Adherent and Suspension
Cell MorphologyRound|Polygonal
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetOther
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line isPrigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

Note: This cell line require horse serum to maintain cells in suspension. Once fetal bovine serum is used, the cells cannot be grown in horse serum again for cell suspension.
QC1) 400 µg/ml neomycin (G418) for selection; 2) Western blot was preformed for gp120.
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorDana-Farber Cancer Institute Inc
Documents


Supporting Protocol
MSDS

    QC

      Other

      FAQs


      There are no FAQs for this product yet!
      References


      2
      • Terwilliger, E et al. "Cell lines that express stable env gene products from three strains of HIV-1" Journal Acquired Immune Defic Syndr 1:317-323 (1988).
      • Rosen, CA et al. "Construction of recombinant murine retroviruses that express the human T cell leukemia virus type II and human T cell lymphotropic virus type III trans-activator genes" J Virol 57:379-384 (1985). PubMed: 3001360.