E. coli CRISPR Knockout Teaching Kit

The E. coli CRISPR Knockout Teaching Kit allows students to gain hands-on laboratory experience utilizing CRISPR - a revolutionary gene-editing technique. In this robust experiment, the student performs a gene knockout of a genomically-encoded chloramphenicol-resistance cassette employing CRISPR and lambda red recombineering (see our Knockout Case Study for details). This results in E. coli that is now sensitive to the antibiotic chloramphenicol – a phenotype that can be easily screened for. The experiment is carried out in a safe laboratory strain of E. coli (BSL-1) and uses standard microbiological/molecular biology reagents and equipment allowing for ease of implementation in most teaching labs.

For more information, please contact [email protected].

• Students gain hands-on laboratory experience using the CRISPR gene-editing technique. 
• Experiment performed in E. coli 
• Simple and robust editing occurs in 4 days 
• Uses standard reagents and equipment

This kit is intended for teaching purposes only.