E. coli CRISPR Knockout Teaching Kit

CAT.NOUNITPRICE
K09915 Reactions
$425.00

Specifications


Description

The E. coli CRISPR Knockout Teaching Kit allows students to gain hands-on laboratory experience utilizing CRISPR - a revolutionary gene-editing technique. In this robust experiment, the student performs a gene knockout of a genomically-encoded chloramphenicol-resistance cassette employing CRISPR and lambda red recombineering (see our Knockout Case Study for details). This results in E. coli that is now sensitive to the antibiotic chloramphenicol – a phenotype that can be easily screened for. The experiment is carried out in a safe laboratory strain of E. coli (BSL-1) and uses standard microbiological/molecular biology reagents and equipment allowing for ease of implementation in most teaching labs.

For more information, please contact [email protected].

SKUK099
Unit quantity15 Reactions
Applications
  • Students gain hands-on laboratory experience using the CRISPR gene-editing technique. 
  • Experiment performed in E. coli 
    • Simple and robust editing occurs in 4 days 
    • Uses standard reagents and equipment
Note

This kit is intended for teaching purposes only.

Documents


Supporting Protocol
MSDS

    QC

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