iPSC Reprogramming

One of the major goals of iPSC research is for disease modeling and novel therapeutic (clinical) applications. To simplify iPSC induction, scientists at ABM have successfully developed a broad line of iPSC reprogramming factors including proteins, lysates, lentiviruses, adenoviruses, minicircle DNA and cord blood episomal iPSC vectors.

Scientists at abm Inc. provide researchers with the most comprehensive reprogramming vectors for iPSC research. Every vector has been sequenced and vigorously tested to be functional. With those tools readily available, research scientists can now focus on their research projects without having to spend time on the development of basic vectors of their own. Preliminary customer feedback has shown that the episomal plasmids we designed and lentivirus we produced are also significantly more efficient in reprogramming fibroblasts from mice, rats and humans than other similar vectors on the market.

• Human and mouse recombinant iPSC proteins in either crude cell lysate or purified protein format completely eliminates genome manipulation associated with vector-based iPSC factors.
• Comprehensive selection of iPSC lentiviral vectors and viruses with choice of different promoters, plus a polycistronic iPSC lentivector or lentivirus containing all four transcription factors in the Thomson (OSNL) Protocol.
• Adenovirus formats for highly efficient, non-integrating transient transduction in most cell lines, based on human adenovirus type 5 and prepared in high titer format for immediate applications.
• Minicircle DNA, available with either a CMV or EF1α promoter and a GFP reporter for transgene expression, is the only truly xeno-free plasmid without any sequence of bacterial or viral origin and is ideal for xeno-free iPSC reprogramming.
• Using oriP/EBNA1 (Epstein-Barr nuclear antigen-1)-based episomal vectors have been shown to be effective in the reprogramming of human cord blood cells (CB) or peripheral blood (PB) cells, presenting great potential applications in future regenerative medicine due to its easy accessibility as compared to isolation of same type of cells from bone marrow.
• Licensed from the University of Toronto, highly sensitive and universally applicable EOS lentiviral and adenoviral vectors are available for immediate iPSC identification. EOS (Early Transposon promoter and Oct-4 and Sox2 enhancers) element is a newly identified promoter that only functional in stem cells, but not differentiated cells, thus usful as a reporter promoter for stem cell identification.