miR-Tuners

Conventional mammalian expression systems rely on the usage of strong viral promoters (e.g. CMV and SV40) which in turn over-express the gene of interest (GOI). However, over-expression of a foreign gene can lead to detrimental effects in the cell and importantly, fails to replicate normal physiological gene expression levels. To address this issue, miR-Tuners can be used to precisely fine-tune transgene expression levels in a predictable manner.

miR-Tuners are short, synthetic miRNA response element sequences which reside in the 3’ UTR of the GOI. These sequences are responsive to a native miRNA species which is widely expressed in many human and mouse cells. Using the miR-Tuner Low, Med or Hi will allow for precise low, medium or high expression levels of the GOI, respectively.

abm offers a variety of miR-Tuner products which can precisely and predictably control transgene expression levels in mammalian cells. These products include ready-to-clone miR-Tuner MCS (multiple cloning site) Lentiviral Vectors and custom cloning services of your GOI into a set of miR-Tuner Vectors and/or Lentiviral packaging.


Applications

Fine-tuning transgene expression levels in mammalian cells for:

  • Engineered genetic circuits and cell lines
  • Studying physiological and biochemical changes

Browse Products

4 Items

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  1. Price: $750.00
    Unit: 3 x 10 µg
    The Set of 3 MCS vectors contain a multiple cloning site (MCS) and reporter gene eGFP linked via the 2A peptide. This construct allows for cell sorting by flow cytometry in order to isolate cells with the desired expression levels. These vectors...


  2. Price: $850.00
    Unit: 3 x 200 µl
    The Set of 3 Control Lentiviruses are ready-to-transduce packaged Lentiviral Vectors LV104-Low, Med and Hi. The miR-Tuner element is cloned within the 3’UTR of the reporter gene Firefly Luciferase. This allows for simple validation of the...


  3. Price: $350.00
    Unit: 3 x 10 µg
    The Set of 3 Control vectors contains Low, Med or Hi miR-Tuner elements cloned within the 3’UTR of the reporter gene Firefly Luciferase. This allows for simple validation of the miR-Tuner system by measuring and comparing output luminescence....


  4. Price: $850.00
    Unit: 3 x 10 µg
    The Set of 3 MCS vectors contain a multiple cloning site (MCS) and reporter gene eGFP linked via the 2A peptide. This construct allows for cell sorting by flow cytometry in order to isolate cells with the desired expression levels. These vectors...

4 Items

per page
Set Descending Direction