Mycoplasma Elimination Cocktail
|G398||1.0 ml (3.5 mg/ml)|
abm’s Mycoplasma Elimination Cocktail is a specially formulated agent to effectively clear cells from Mycoplasma contamination in both adherent and suspension cells. Mycoplasma contamination is currently the most prevalent contamination in all cell culture facilities around the world.
|Usage||• Direct addition of cocktail to cell culture medium|
• Effectively eliminates over 70 types of mycoplasma species
• Not cytotoxic to most mammalian cell lines.
Store at -20°C for long term storage. 4°C for short term use.
|Unit quantity||1.0 ml (3.5 mg/ml)|
|What is the basis of the positive control in the mycoplasma kit?|
It is DNA fragments of Mycoplasma gDNA diluted in medium.
|Can you send the protocol for this kit, please? I am interested in purchasing this product, but I am not sure how much cells I can purify with this kit. I just detected mycoplasma with your mycoplasma detection kit. Thank you for your help.|
Here is the link of protocol. http://www.abmgood.com/Mycoplasma-Elimination-Cocktail-G398.html
|I notice a white precipitate after the delivery of the product; is this normal?|
Yes, it is normal to observe a white precipitate in each vial. Please re-suspend the vial to mix the components. The solution is particulate in nature so it is not homologous nor will the particles dissolve. There should be divided solids throughout the tube after mixing. If vortexing doesn't help, you can try pipetting the mixture inside the hood if the pellet is hard to break.
|Which mycoplasma strains are targeted?|
Potential strains which may be affected are: Mycoplasma pneumonia strains Mycoplasma hyosynoviae strains Mycoplasma hyorhinis strains Mycoplasma synoviae strains Note: Some strains may naturally become tolerant to this cocktail due to their endogenous defense mechanisms, and thus may show reduced sensitivity. In such cases, other antibiotic combinations may need to be considered.
|I have used the Mycoplasma Elimination Cocktail on my cells but my cells are dying. Why is this happening?|
The mycoplasma cocktail is a very potent combination of different antibiotics to increase the success of eliminating mycoplasma. Even though we have tested and confirmed no cytotoxicity was observed for a variety of cell lines, we would still recommend optimizing the best dilution for your specific cell line in order to achieve best results.
|There is a ~200-250bp band in one of my samples, does this mean it is mycoplasma positive?|
In rare cases, there may be a weak non-specific binding to a region of human gDNA due to the degenerate nature of the primers that can amplify a weak 250bp band in human cell samples. Your sample can be considered as negative for mycoplasma, as mycoplasma amplicons will be 370-550bp in size. You can try to increase the annealing temperature of the PCR program to 60°C to reduce this non-specific amplification.
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