Ni-IDA Agarose Resins

Cat. No.
G253
Unit
100 ml
Price
$1235.00
Cat. No. G253
Name Ni-IDA Agarose Resins
Unit 100 ml
Category Buffers & Chemicals
Description
Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni2+ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.
 
Special Features
  • One-step easy purification protocol from crude lysate to >95% pure protein.
  • Purification under native or denaturing conditions.
  • Ready-to-use for any scale of purification.
  • Protein binding capacity: approx. 20-40 μmol Ni2+/ml gel.
  • Economical value.
  • Can be used for successive cycles.
  • Resins and columns formats suitable for batch and gravity purification.
Application
  • Purification of His-tagged proteins.
  • Antibody purification.
  • Assays involving protein-protein and protein-DNA interactions.
  • Structural and functional investigation of proteins.
Storage Condition

This product is stable at cool, dry, room temperature. For extended storage, keep at 4°C. The expiration date is one (1) year from the date of receipt.

Guarantee

abm guarantees that all our Tissue Slides will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G253
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