NOD1 Stable Knockout AGS (41H8) Cell Line
|T6188||1x106 cells / 1.0 ml|
CRISPR/Cas9 gene editing was used to generate NOD1 KO clones. AGS cells were transformed with vectors expressing Cas9 nickase nuclease and pairs of guide RNAs (gRNAs) specific for the NOD1 gene. NOD1 Stable Knockout AGS (41A8) Cell Line (available at abm; Cat. No. T6187) and NOD1 Stable Knockout AGS (41H8) Cell Line were confirmed as having 71-bp and 31-bp deletions within the coding regions of the caspase activation and recruitment domain (CARD) of NOD1. This cell line had been shown to have defective CXCL8 responses to stimulation with NOD1 ligands. Control cells are also available abm; Cat. No. T8246 to use alongside with the cells.
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Donor Disease||Stomach adenocarcinoma|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||Puromycin resistance|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) PCR; 2) Pyrosequencing|
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