NOD1 Stable Knockout AGS (41H8) Cell Line

CAT.NOUNITPRICE
T61881x106 cells / 1.0 ml
$0.00

Specifications


Description

CRISPR/Cas9 gene editing was used to generate NOD1 KO clones. AGS cells were transformed with vectors expressing Cas9 nickase nuclease and pairs of guide RNAs (gRNAs) specific for the NOD1 gene. NOD1 Stable Knockout AGS (41A8) Cell Line (available at abm; Cat. No. T6187) and NOD1 Stable Knockout AGS (41H8) Cell Line were confirmed as having 71-bp and 31-bp deletions within the coding regions of the caspase activation and recruitment domain (CARD) of NOD1. This cell line had been shown to have defective CXCL8 responses to stimulation with NOD1 ligands. Control cells are also available abm; Cat. No. T8246 to use alongside with the cells.

SKUT6188
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemStomach
Cell MorphologyEpithelial-like
ApplicationsFor Research Use Only
Donor DiseaseStomach adenocarcinoma
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfilePuromycin resistance
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) PCR; 2) Pyrosequencing
Documents


Supporting Protocol
MSDS

    QC

      Other

      FAQs


      There are no FAQs for this product yet!
      References


      There are no references for this product yet!