Opti-Protein Marker

Cat. No.
G252
Unit
500 μl/100 loads
Price
$110.00
Cat. No. G252
Name Opti-Protein Marker
Unit 500 μl/100 loads
Category Gels, Blots & ELISAs
Description

Opti-Protein Marker/Ladder consists of 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. This unique protein ladder allows you to monitor protein separation during electrophoresis, estimate molecular weight of the protein of interest, and evaluate western blot transfer efficiency. It also includes ~10, ~40 and ~90 kDa reference bands coupled with blue dye that allow easy tracking and identification.

Number of Bands          11

Protein Range                10kDa-175kDa

Suggested Load             Mix gently by pipetting up and down a few time, then directly load 5 μl into well of gel.

Application

• Monitoring protein migration during SDS-PAGE
• Monitoring efficiency of protein transfer onto PVDF or nitrocellulose for Western Blotting
• Sizing of proteins on SDS-PAGE and Western Blots

Storage Condition

Store @ 4°C for 3 months or -20°C for 24 months.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G252
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Supporting Protocol
  • Li, HL et al. "Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer" Oncology Letters 5:1989-1996 (2015). DOI: 10.3892/ol.2015.2984. Application: Ladder.
  • Sharma, V et al. "Registered report: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation" eLIFE 5 (e11414): (2016). DOI: 10.7554/eLife.11414. Application: Western Blot.
  • Takci, HAM et al. "Extracellular Pectinase Production and Purification from a Newly Isolated Bacillus subtilis Strain" International Journal of Food Properties 19 (11):2443-2450 (2016). DOI: 10.1080/10942912.2015.1123270. Application: SDS-PAGE.
  • Sharma, V et al. "Registered Report: Diverse somatic mutation patterns and pathway alterations in human cancers" eLIFE 5 (e11566): (2016). DOI: 10.7554/eLife.11566. Application: Western Blot.
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