pLenti-Beta actin-5'UTR-Dual-Luciferase Control Vector

Cat. No.
m036
Unit
1.0 μg
Price
$175.00
Cat. No. m036
Name pLenti-Beta actin-5'UTR-Dual-Luciferase Control Vector
Unit 1.0 μg
Category Control Vectors & Viruses
Description

A blank control vector. This vector has a beta actin promoter driving expression of firefly luciferase (P. pyralis). A second Renilla (R. reniformis) luciferase is driven by the SV40 promoter to allow for normalization.

Vector Map pLenti-5-UTR-Dual-Luc-Blank (click blue link to view)
List of Markers Bacterial: Kanamycin Mammalian: Puromycin, Luciferase
Promoter b-actin, SV40
Storage Condition

1 year when stored at -20°C or lower in a non-frost free freezer.

Note

Every gene has a 5’ UTR and 3’ UTR (Untranslated Region) upstream and downstream, respectively, of the coding region. abm’s 5’ UTR Reporter Vectors consist of the 5’ UTR of the gene of your choice preceding a GFP or Luciferase reporter, driven by a β-actin promoter. These vectors are ideal for studying the regulation of translation by 5’ UTR elements.

The 5’ UTR reporter constructs can be combined with our Site-Directed Mutagenesis Service (Cat. No. C047) in order to define RBP or miRNA binding sites, or investigate the effects of uAUGs or uORFs on gene expression. To use alongside our custom 5’ UTR Vectors, we offer this blank 5’ UTR Vector (negative control). 

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. m036
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