Pro Ligation-Free Cloning Kit (20 reactions)

The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs.

Key Kit Features:

• One-step cloning: No more time-consuming, multi-step traditional cloning that depnds on available restrict sites or compatible ends
• Scar-free cloning: No unwanted/extra base pairs in the inserts
• Easy-to-customize: Clone in promoter, gene, tag, multi-fragment or whiole vector constructs in one step
• Ready for downstream applications: Ligase-free reaction, no gel extraction or column purification steps needed
• Robust assembly: Better performance for difficult, multi-fragment assemblies such as CRISPR multiplex sgRNA vector assembly
• Increased thermostability: High colony yields

Testimonial:

"I tried the molecular cloning of inserting two DNA fragments (2Kb & 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit."

- Jianfeng Lan, The Buck Institute

• Rapid multi-fragment cloning and assembly of whole vector constructs
• Clone in a promoter, gene, and tag, all in one step
• Rapid assembly of CRISPR multiplex gRNA vectors in one step