Pro Ligation-Free Cloning Kit (20 reactions)
CAT.NO | UNIT | PRICE |
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E086 | 20 Reactions | $100.00 |
Description | The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs. Key Kit Features:
Testimonial: "I tried the molecular cloning of inserting two DNA fragments (2Kb & 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit." - Jianfeng Lan, The Buck Institute |
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SKU | E086 |
Unit quantity | 20 Reactions |
Applications |
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Supporting Protocol
MSDS
QC
Other
1
- Sherman, S. L. . "Cofactor-Independent Pinacolase Directs Non-Diels-Alderase Biogenesis of the Brevianamides" : ().