Pro Ligation-Free Cloning Kit (20 reactions)
The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs.
Key Kit Features:
"I tried the molecular cloning of inserting two DNA fragments (2Kb & 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit."
- Jianfeng Lan, The Buck Institute
|Unit quantity||20 Reactions|
- Sherman, S. L. . "Cofactor-Independent Pinacolase Directs Non-Diels-Alderase Biogenesis of the Brevianamides" : ().