Question (1131)
Question
Is a kozak sequence required for mammalian gene expression?
ABM community
Verified customer
Asked on Nov 13 2025
Answer
Yes, a Kozak sequence (GCCGCCACCATGG) immediately upstream of the ATG is required to ensure optimal protein translation. This is true for all mammalian expression vectors that we offer. Please note that blank vector/viruses do not express anything so the Kozak sequence will be absent.
ABM Scientific Support
Answered on Nov 13 2025