Question (156)
Question
How do I know if abm’s lentivirus is replication defective?
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Lentiviral vector stocks are tested for the presence of replication-competent lentivirus (RCL) by monitoring p24 antigen expression in the culture medium of transduced 293T cells for 30 days. Since the vectors are replication-defective, no amplification of the p24 expression would normally be expected. However, if there were an increase in P24 signal over time, it would be indicative of RCL contamination. The p24 expression is assayed by qPCR.
ABM Scientific Support
Answered on Mar 24 2025