Question (157)
Question
How efficient is lentivirus transduction?
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Transduction is highly dependent on the cell line or cell type. In general, lentivirus has high transduction efficiency in most cell lines with up to 100% efficiency in HEK293 and other commonly used cell lines. The lowest efficiency observed is 10%. We recommend customers evaluate transduction efficiency for a specific cell line by using a control GFP lentivirus. For customers that aim to generate a stable cell line, 1% transduction efficiency is enough as every transduced cell is permanently integrated with the vector. Therefore, 10ml of 10^6 cfu/ml lentivirus would result in more than enough transduced cells.
ABM Scientific Support
Answered on Mar 24 2025