Question (161)
Question
Following lentivirus infection into my target cells I noticed that the cell’s have a shorter life span compared to my uninfected cells. Why?
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The supernatant is collected from the viral production stage and this is used to infect the target cells. DMEM media is utilized during production and often at the time of harvesting it has been depleted of nutrients. Because of this the supernatant may create a stressful environment for the target cells or DMEM may be unsuitable for your cell line.
ABM Scientific Support
Answered on Mar 24 2025