Question (2027)
Question
During lentivirus production, does only the gene of interest get incorporated into the capsid of lentivirus?
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Asked on Mar 24 2025
Answer
During the process of lentivirus packaging, the genes (including the gene of interest) between the 5'-LTR and 3'-LTR of the transfer plasmid (i.e. lentivector DNA) will be incorporated into the packaged recombinant lentivius. The packaging plasmids are provided separately from the transfer plasmid, and their purpose is to provide pseudotyping and structural protein instructions only. They will not be incorporate into the capsid.
ABM Scientific Support
Answered on Mar 24 2025