Question (2038)
Question
I bought abm’s siRNA vector and virus. Transfection resulted in successful gene knockdown but the transduction did not demonstrate the same efficiency of gene knock down. Why?
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Transfection of cell lines such as 293T can achieve very high efficiency – near 100%. Thus it is possible to achieve good siRNA gene knock down via transfection. However, you will not have a stable cell line. With lentivirus, you will need to establish a stable cell line where 100% of the cells express the siRNA. Without a pure stable cell line, you will not see efficient gene knock down effects.
ABM Scientific Support
Answered on Mar 24 2025