Question (2238)
Question
Can you recommend a specific reagent and protocol to immortalize my cell line?
ABM community
Verified customer
Asked on Aug 11 2025
Answer
Successful cell immortalization is unpredictable and must be determined experimentally, as results vary with species, tissue origin, growth characteristics and whether or not specific cell functions need to be preserved.
Our in-house experiments using the SV40T and hTERT lentiviruses have achieved the highest immortalization success rates in a variety of different cell types.
Our in-house experiments using the SV40T and hTERT lentiviruses have achieved the highest immortalization success rates in a variety of different cell types.
ABM Scientific Support
Answered on Aug 11 2025