Question (2332)
Question
What are CRISPR activation & repression vectors and how do they work?
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Asked on Jan 30 2026
Answer
These constructs are a collection of lentiviral vectors that express sgRNAs targeting the upstream 5′ UTR of a specific gene. When combined with dCas9-VPR, they mediate targeted gene activation by recruiting transcriptional activators. In contrast, pairing the sgRNAs with dCas9-KRAB results in targeted gene repression through KRAB-mediated transcriptional silencing.
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Answered on Jan 30 2026