Question (512)
Question
Does vector pLenti-GIII-CMV contain recombination sites flanking the insert sequence?
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Verified customer
Asked on Mar 24 2025
Answer
Yes, there is an extra ~125bp to either side of the CDS insert. Thus, if the vector is digested using EcoRV restriction enzyme the resulting DNA band will be the insert size plus 250bp.
ABM Scientific Support
Answered on Mar 24 2025