qPCR AAV Titer Kit

CAT.NOUNITPRICE
G931100 rxn
$195.00

Customize qPCR AAV Titer Kit
1 x BlasTaq™ 2X qPCR Titer MasterMix   + $0.00
1 x Primer Mix   + $0.00
1 x Control DNA   + $0.00
2 x DNase I Reaction Mix   + $0.00
1 x ROX Reference Dye   + $0.00
2 x Nuclease-Free H2O   + $0.00

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Your Customization
qPCR AAV Titer Kit
qPCR AAV Titer Kit

In stock

$195.00

Summary
    Specifications


    Description

    abm’s qPCR AAV Titer Kit is a one-step assay by qPCR which does not require additional lysis, and can be completed under one hour. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.

    Product ComponentQuantity
    BlasTaq™ 2X qPCR Titer MasterMix 1.25 ml
    Primer Mix 100 rxn (200 µl)
    Standard Control DNA 50 µl
    DNase I Reaction Mix 2 x 1.0 ml
    ROX Reference Dye 15 µl
    Nuclease-Free H2O 2 x 1.0 ml


     

    SKUG931
    Unit quantity100 rxn
    Note

    qPCR AAV Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

    MACHINE TYPEMACHINE
    High ROX Machines
    • ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
    • Biometra TOptical
    • Fluidigm BioMark™
    • Wafergene SmartChip System
    • TianLong TL998 System
    Low ROX Machines
    • ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
    • BioGene InSyte™
    • Illumina Eco
    • Analytikjena qTower Series
    • Stratagene® Mx3000, Mx3005, Mx4000
    No ROX machines
    • BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
    • Roche LightCycler® (480, 1536, Nano)
    • MJ Research Opticon™, Opticon™ 2, Chromo® 4
    • Eppendorf™ Realplex 4
    • BioGene SynChron™
    • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
    • Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
    • Cepheid SmartCyler, GeneXpert
    • Enigma™ ML
    • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
    • Qiagen Rotor-Gene™ (Q, 6000)
    • Takara Dice™
    • Thermo Scientific PikoReal
    • DNA-Technology DT96, DTlite, DT-322
    • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
    • Bioneer Exicycler™
    Storage Condition

    Store at -20°C.

    Documents


    Supporting Protocol
    MSDS
    QC

      Other
      FAQs


      How is the virus lysed?
      The virus is denatured during the initial heating step of the qPCR (i.e., Enzyme Activation, 95°C).
      What samples were used to create the standards in this kit?
      Plasmid with partial virus DNA were used to generate the standards.
      Is this kit able to detect all AAV serotypes?
      Yes, this kit should be able to detect all AAV serotypes. If in doubt, we can also verify for you if you can send us your ITR sequences.
      References


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