Rat iPS Cell Culture Medium Kit
|Description||The Rat iPSC Culture Medium Kit is formulated and optimized for the long-term undifferentiated expansion of rat induced pluripotent stem cells or rat embryonic stem cells (ESCs). The Rat iPSC Culture Medium Kit has 2 components, the basal medium and the growth supplements to allow flexibility for daily culture. ABM’s Rat iPSC Culture Medium supports proliferation and maintains multi-lineage differentiation potentials of rat iPSC.|
|Caution||For research use only and is not intended for therapeutic or diagnostic applications. abm products are intended for laboratory research purposes only, unless noted otherwise. They are not intended for use in humans. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.|
|Sterility||Each lot is tested for the ability to promote proliferation of Rat iPSC cells. All components are endotoxin free and negative for bacteria, yeast and fungi.|
|Storage Condition||Store Basal Medium at 2-8°C and Growth Supplements at -20°C.|
|Unit quantity||1 Kit|
|How much serum is in the media?|
There is 5-7% of FBS in the media. Serum does not need to be added.
|What is the protocol for freezing iPSCs?|
1) 4-5 days after cell split, when iPSCs reach a confluency of 30-60%, aspirate out spend medium and add 1ml 0.5mM EDTA/PBS. Incubate cells at 37C for ~5min. 2) When colonies start to detach, gently harvest all the cells into a 5-ml or 15-ml or 50-ml tube by pippeting 1-2 times. Avoid multiple pippetting. Breaking down clumps into single cells may substantially decrease cell survival. May add iPSC medium to harvest all the cells. 3) Spin down at 200g for 2-3 minutes. 4) Carefully aspirate out supernatent and gently resuspend cells in 0.5ml iPSC medium. 5) Add 0.5ml freezing medium (TM023) and ROCK inhibitor. Mix well by flipping the vial and transfer the medium to a cryovial. 6) Finally transfer the vial to -80C freezer for short-term storage (days to weeks). After cells are frozen, you may then transfer the vial to liquid nitrogen tank for long-term storage (years).
|I have contaminated my medium, what should I treat contaminations with?|
You may consider filtering the medium again. Add primocin to the culture for 2 weeks. Before nuclefection, heat the plasmids at 50C for 5 minutes. Please note that this is a suggestion only and there are no guarantees.
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