Recombinant Human GDNF (E. coli)
|Description||GDNF is a disulfide-linked, homodimeric neurotrophic factor that belongs to the cystine-knot family of growth factors. Natively glycosylated, GDNF signals through a multi-component receptor system that comprises of a RET and one of the four GFRα receptors. In embryonic midbrain cultures, GDNF promotes the survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity Recombinant Human GDNF is a non-glycosylated, disulfide-linked homodimer (30 kDa) consisting of 135 amino acids in each subunit.|
|Aliases||GDNF, Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor, ATF|
Recombinant Human Glial-Derived Neurotrophic Factor (GDNF)
|Species||Human (H. sapiens)|
|Molecular Weight||15 kDa|
|Endotoxin Level||<1.0 EU/µg of recombinant protein as determined by the LAL method|
|Purity||>95% as determined by SDS-PAGE|
|Formulation||Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl (pH 4.0)|
|Function||The ED50 as determined by the proliferation of rat C6 cells was found to be <0.1 ng/mL|
|Reconstitution||A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.|
|Storage||The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable lo|
|Usage||For research use only. Not for diagnostic or therapeutic use.|
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- Role of Growth Factors in Cell Differentiation (Poster)
|How are endotoxin levels measured?|
1. For estimating the endotoxin levels; we use the LAL (Limulus Amebocyte Lysate) method: The lysate from horseshoe crab amebocytes clots in the presence of very low endotoxin. This reaction is the basis of the Limulus amebocyte lysate (LAL) assay which was approved by the FDA in 1970. · Endotoxin is generally measured in Endotoxin Units per milliliter (EU/mL). · For recombinant proteins: EU is reported per microgram of protein. · One EU = 0.1-0.2 ng endotoxin/µg of protein. · At abm, we do the LAL chromogenic assays that can detect down to 0.01 EU/ml.
|With regard to the BSA levels in some Growth Factors and Cytokines, can you please provide an explanation as to why they are so high?|
The amount of BSA, as part of the formulation of a protein, can vary considerably depending on how much BSA was deemed optimum/necessary for protein stability in combination with /in-lieu of - other possible additives. The aforementioned formulations are somewhat analogous to the “carrier” versions of many formulations from “R and D systems” that have as high as 50 µg of BSA per µg of the recombinant protein product. If, needed or desired, abm scientists can substitute BSA for other stabilizing additives for most formulations.
|Are your Escherichia coli sourced growth factors: 1) Human derived materials free? 2) Recombinant proteins free?|
Yes, all of abm's growth factors made in Escherichia coli using recombinant technology contain no human derived-products or other recombinant proteins. In the rare cases of BSA presence, this will be mentioned in the product's formulation.
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