RNAifectin™
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RNAifectin™ Transfection Reagent
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Cat.No.: G073
Quantity: 1.0 ml (100-500 transfections)
Stock: In Stock
Price: $165.00
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Data Sheet
Description Especially developed for the efficient transfection of eukaryotic cells with RNAi oligo's. This transfection reagent is perfect for any of your RNAi transfection needs.
Application RNAi knockdown experiments in a wide variety of cell lines (HeLa, HEK-293 etc). Highly specific and non-toxic transfection.
Protocol Click To Open
Storage Store at 4ºC. Do not freeze.
Notes This product is distributed for laboratory research only. Caution: Not for diagnostic use .
Product Documents
Product References
FAQs
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Does freeze-thaw affect the performance of the transfection reagent?
Freeze-thaw does damage the transfection reagent slightly, at an approximate 5% loss.

If possible, test the agent with a GFP control first.
In your protocol there is no mention of 24 well or 12 well plates. Is the transfection still efficient if performed in such plates. If yes, what would be the quantities required to ensure a successful transfection.
The transfection step is a harsh and toxic environment for cells to be exposed to, and it is common to see some cell death as a result. Therefore we would recommend carrying out this step in 6-well plates at a minimum to ensure a good efficiency.